Detection of cytotoxic anti-LEDGF autoantibodies in atopic dermatitis

Masahiko Ayaki, Nobuyuki Ohoguro, Noriyuki Azuma, Yoshinao Majima, Kiyomi Yata, Nobuhiro Ibaraki, Dhirendra P. Singh, Vincent Ko, Toshimichi Shinohara

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

In the last two decades, atopic dermatitis (AD) has been of increasing clinical significance in Japan. Eight-20% of patients with AD developed progressive cataracts (cataract-AD) and lens epithelial cells (LECs) were severely damaged. Lens epithelium-derived growth factor (LEDGF) is a newly isolated survival factor. In the presence of LEDGF, LECs survive well and in the absence of LEDGF, they become highly susceptible to stress. We investigated (1) whether auto-antibody (auto-Ab) to LEDGF is present in sera of AD patients and (2) whether depletion of LEDGF by the auto-Ab kills LECs. In sera from 26 patients with AD using ELISA, we found significantly higher levels of auto-Ab to LEDGF than that in a normal control group. Affinity purified auto-Ab to LEDGF from these sera killed LECs without complement activation. Levels of histamine in the AD group were significantly higher and levels of prostaglandin E2 were significantly lower than in the normal group. However, statistically there are no differences between sera from AD and cataract-AD in levels of Ab to LEDGF, histamine, prostaglandin E2 (PGE2), immunoglobulin E (IgE) and eosinophiles. We speculate that cataract-AD may be induced, in part, by a combination of high levels of serum histamine and eye rubbing which could break the blood-aqueous barrier to allow the entry of Ab to LEDGF into the privileged compartment, thus, reducing LEDGF levels, resulting in damage to LECs, and cataract formation.

Original languageEnglish (US)
Pages (from-to)319-327
Number of pages9
JournalAutoimmunity
Volume35
Issue number5
DOIs
StatePublished - Aug 1 2002

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Keywords

  • Atopic dermatitis
  • Auto-Ab to LEDGF
  • Cataract
  • Histamine
  • Lens epithelium-derived growth factor (LEDGF) and cell death

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ayaki, M., Ohoguro, N., Azuma, N., Majima, Y., Yata, K., Ibaraki, N., Singh, D. P., Ko, V., & Shinohara, T. (2002). Detection of cytotoxic anti-LEDGF autoantibodies in atopic dermatitis. Autoimmunity, 35(5), 319-327. https://doi.org/10.1080/0891693021000003198