Detection of cardiac myosin heavy chain-α-specific CD4 cells by using MHC class II/IAk tetramers in A/J mice

Chandirasegaran Massilamany, Arunakumar Gangaplara, Nora M Chapman, Noel Rose, N R Jayagopala Reddy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with cardiac myosin heavy chain (Myhc)-α 334-352, whereas B10.A mice carrying a similar allele IAk are relatively resistant, suggesting that the generation of Myhc-α-reactive T cell repertoires is influenced by genetic background. To enumerate the precursor frequencies of Myhc-α-specific CD4 T cells, we sought to create IAk tetramers for Myhc-α 334-352. Tetramers were created using approaches that involve covalent tethering of individual peptide sequences or exogenous loading of peptides into empty IAk molecules by peptide-exchange reaction. Using ribonuclease 43-56 tetramers as controls, we demonstrated that by flow cytometry (FC), Myhc-α 334-352 tetramers specifically bind myosin-reactive T cells. CD4 cells isolated from A/J mice immunized with Myhc-α 334-352 were used to optimize conditions for tetramer staining, and neuraminidase treatment prior to tetramer staining permitted the detection of Myhc-α-specific cells ex vivo. The reagents are useful tools for monitoring the frequency of Myhc-α-reactive CD4 cells and to determine their pathogenic potential at a single cell level by FC.

Original languageEnglish (US)
Pages (from-to)107-118
Number of pages12
JournalJournal of Immunological Methods
Volume372
Issue number1-2
DOIs
StatePublished - Sep 30 2011

Fingerprint

Cardiac Myosins
Myosin Heavy Chains
T-Lymphocytes
Peptides
Flow Cytometry
Alleles
Staining and Labeling
Myocarditis
Neuraminidase
Myosins
Ribonucleases

Keywords

  • Autoimmunity
  • Cardiac myosin
  • MHC class II tetramers
  • Mouse model
  • Myocarditis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Detection of cardiac myosin heavy chain-α-specific CD4 cells by using MHC class II/IAk tetramers in A/J mice. / Massilamany, Chandirasegaran; Gangaplara, Arunakumar; Chapman, Nora M; Rose, Noel; Reddy, N R Jayagopala.

In: Journal of Immunological Methods, Vol. 372, No. 1-2, 30.09.2011, p. 107-118.

Research output: Contribution to journalArticle

Massilamany, Chandirasegaran ; Gangaplara, Arunakumar ; Chapman, Nora M ; Rose, Noel ; Reddy, N R Jayagopala. / Detection of cardiac myosin heavy chain-α-specific CD4 cells by using MHC class II/IAk tetramers in A/J mice. In: Journal of Immunological Methods. 2011 ; Vol. 372, No. 1-2. pp. 107-118.
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