Detection of bovine leukemia virus in b-lymphocytes by the syncytia induction assay

Prem S. Paul, Kem A. Pomeroy, Anthony E. Castro, Donald W. Johnson, Charles C. Muscoplat, Dale K. Sorensen

Research output: Contribution to journalArticle

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Abstract

Bovine peripheral blood lymphocytes (PBL's) from 3 cows and 1 steer infected with bovine leukemia virus (BLV) were separated by fractionation through nylon wool columns into nylon-adherent and nonadherent cell populations. Nylon-adherent cells were enriched in B-lymphocytes, as determined by the presence of surface membrane immunoglobulins (slg), whereas nylon-nonadherent cells or “non-B-lymphocytes” contained few slg-bearing cells. PBL’s and separated B-and non-B-lymphocyte populations were assayed for the presence of BLV by the induction of syncytia in bovine embryonic spleen cells. PBL’s and B-lymphocyte populations both produced many syncytia, whereas non-B-lymphocytes yielded few or no syncytia. The specificity of syncytia induction for BLV was demonstrated by neutralization of syncytia formation by anti-BLV serum. PBL’s from 2 control animals were negative for syncytia induction. This study presents further evidence that B-lymphocytes are the target cells for BLV infection.

Original languageEnglish (US)
Pages (from-to)1269-1272
Number of pages4
JournalJournal of the National Cancer Institute
Volume59
Issue number4
DOIs
StatePublished - Oct 1977

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Bovine Leukemia Virus
Giant Cells
Nylons
Lymphocytes
B-Lymphocytes
Population
Virus Activation
B-Cell Antigen Receptors
Wool
Virus Diseases
Spleen
Membranes
Serum

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Paul, P. S., Pomeroy, K. A., Castro, A. E., Johnson, D. W., Muscoplat, C. C., & Sorensen, D. K. (1977). Detection of bovine leukemia virus in b-lymphocytes by the syncytia induction assay. Journal of the National Cancer Institute, 59(4), 1269-1272. https://doi.org/10.1093/jnci/59.4.1269

Detection of bovine leukemia virus in b-lymphocytes by the syncytia induction assay. / Paul, Prem S.; Pomeroy, Kem A.; Castro, Anthony E.; Johnson, Donald W.; Muscoplat, Charles C.; Sorensen, Dale K.

In: Journal of the National Cancer Institute, Vol. 59, No. 4, 10.1977, p. 1269-1272.

Research output: Contribution to journalArticle

Paul, PS, Pomeroy, KA, Castro, AE, Johnson, DW, Muscoplat, CC & Sorensen, DK 1977, 'Detection of bovine leukemia virus in b-lymphocytes by the syncytia induction assay', Journal of the National Cancer Institute, vol. 59, no. 4, pp. 1269-1272. https://doi.org/10.1093/jnci/59.4.1269
Paul, Prem S. ; Pomeroy, Kem A. ; Castro, Anthony E. ; Johnson, Donald W. ; Muscoplat, Charles C. ; Sorensen, Dale K. / Detection of bovine leukemia virus in b-lymphocytes by the syncytia induction assay. In: Journal of the National Cancer Institute. 1977 ; Vol. 59, No. 4. pp. 1269-1272.
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