Detection of bovine immunodeficiency virus antibodies in cattle by Western blot assay with recombinant gag protein

Shucheng Zhang, Wenzhi Xue, Charles Wood, Qi Min Chen, Sanjay Kapil, Harish C. Minocha

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A western blot assay using purified recombinant bovine immunodeficiency virus gag protein has been developed for detection of bovine immunodeficiency virus antibodies in bovine serum samples. The test was standardized with known bovine immunodeficiency virus positive and negative bovine serum samples and the monoclonal antibody to gag protein. Both naturally and experimentally infected cattle sera demonstrated positive test results. The result of western blot assay was compared with polymerase chain reaction test results in 134 blood samples collected from Kansas. Twenty-six samples tested positive for bovine immunodeficiency virus DNA with polymerase chain reaction (18.7%) and 25 were positive for the antibody to gag protein by western blot analysis (17.9%). Of 26 cattle testing positive using the polymerase chain reaction assay, 24 were antibody-positive by western blot assay, thus establishing a strong correlation between the two tests. The sensitivity and specificity of western blot relative to polymerase chain reaction are 0.92 and 0.99, respectively. The western blot assay proved to be a specific and sensitive test.

Original languageEnglish (US)
Pages (from-to)347-351
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume9
Issue number4
DOIs
StatePublished - Jan 1 1997

Fingerprint

Bovine Immunodeficiency Virus
Bovine immunodeficiency virus
gag Gene Products
Recombinant Proteins
recombinant proteins
Western blotting
Western Blotting
antibodies
Antibodies
cattle
assays
blood serum
polymerase chain reaction
Polymerase Chain Reaction
testing
Serum
sampling
proteins
DNA-Directed DNA Polymerase
monoclonal antibodies

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Detection of bovine immunodeficiency virus antibodies in cattle by Western blot assay with recombinant gag protein. / Zhang, Shucheng; Xue, Wenzhi; Wood, Charles; Chen, Qi Min; Kapil, Sanjay; Minocha, Harish C.

In: Journal of Veterinary Diagnostic Investigation, Vol. 9, No. 4, 01.01.1997, p. 347-351.

Research output: Contribution to journalArticle

Zhang, Shucheng ; Xue, Wenzhi ; Wood, Charles ; Chen, Qi Min ; Kapil, Sanjay ; Minocha, Harish C. / Detection of bovine immunodeficiency virus antibodies in cattle by Western blot assay with recombinant gag protein. In: Journal of Veterinary Diagnostic Investigation. 1997 ; Vol. 9, No. 4. pp. 347-351.
@article{75fc53c33b53419fa4240da01b1f7990,
title = "Detection of bovine immunodeficiency virus antibodies in cattle by Western blot assay with recombinant gag protein",
abstract = "A western blot assay using purified recombinant bovine immunodeficiency virus gag protein has been developed for detection of bovine immunodeficiency virus antibodies in bovine serum samples. The test was standardized with known bovine immunodeficiency virus positive and negative bovine serum samples and the monoclonal antibody to gag protein. Both naturally and experimentally infected cattle sera demonstrated positive test results. The result of western blot assay was compared with polymerase chain reaction test results in 134 blood samples collected from Kansas. Twenty-six samples tested positive for bovine immunodeficiency virus DNA with polymerase chain reaction (18.7{\%}) and 25 were positive for the antibody to gag protein by western blot analysis (17.9{\%}). Of 26 cattle testing positive using the polymerase chain reaction assay, 24 were antibody-positive by western blot assay, thus establishing a strong correlation between the two tests. The sensitivity and specificity of western blot relative to polymerase chain reaction are 0.92 and 0.99, respectively. The western blot assay proved to be a specific and sensitive test.",
author = "Shucheng Zhang and Wenzhi Xue and Charles Wood and Chen, {Qi Min} and Sanjay Kapil and Minocha, {Harish C.}",
year = "1997",
month = "1",
day = "1",
doi = "10.1177/104063879700900401",
language = "English (US)",
volume = "9",
pages = "347--351",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "4",

}

TY - JOUR

T1 - Detection of bovine immunodeficiency virus antibodies in cattle by Western blot assay with recombinant gag protein

AU - Zhang, Shucheng

AU - Xue, Wenzhi

AU - Wood, Charles

AU - Chen, Qi Min

AU - Kapil, Sanjay

AU - Minocha, Harish C.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - A western blot assay using purified recombinant bovine immunodeficiency virus gag protein has been developed for detection of bovine immunodeficiency virus antibodies in bovine serum samples. The test was standardized with known bovine immunodeficiency virus positive and negative bovine serum samples and the monoclonal antibody to gag protein. Both naturally and experimentally infected cattle sera demonstrated positive test results. The result of western blot assay was compared with polymerase chain reaction test results in 134 blood samples collected from Kansas. Twenty-six samples tested positive for bovine immunodeficiency virus DNA with polymerase chain reaction (18.7%) and 25 were positive for the antibody to gag protein by western blot analysis (17.9%). Of 26 cattle testing positive using the polymerase chain reaction assay, 24 were antibody-positive by western blot assay, thus establishing a strong correlation between the two tests. The sensitivity and specificity of western blot relative to polymerase chain reaction are 0.92 and 0.99, respectively. The western blot assay proved to be a specific and sensitive test.

AB - A western blot assay using purified recombinant bovine immunodeficiency virus gag protein has been developed for detection of bovine immunodeficiency virus antibodies in bovine serum samples. The test was standardized with known bovine immunodeficiency virus positive and negative bovine serum samples and the monoclonal antibody to gag protein. Both naturally and experimentally infected cattle sera demonstrated positive test results. The result of western blot assay was compared with polymerase chain reaction test results in 134 blood samples collected from Kansas. Twenty-six samples tested positive for bovine immunodeficiency virus DNA with polymerase chain reaction (18.7%) and 25 were positive for the antibody to gag protein by western blot analysis (17.9%). Of 26 cattle testing positive using the polymerase chain reaction assay, 24 were antibody-positive by western blot assay, thus establishing a strong correlation between the two tests. The sensitivity and specificity of western blot relative to polymerase chain reaction are 0.92 and 0.99, respectively. The western blot assay proved to be a specific and sensitive test.

UR - http://www.scopus.com/inward/record.url?scp=0031252747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031252747&partnerID=8YFLogxK

U2 - 10.1177/104063879700900401

DO - 10.1177/104063879700900401

M3 - Article

C2 - 9376421

AN - SCOPUS:0031252747

VL - 9

SP - 347

EP - 351

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 4

ER -