Detection of avian pneumovirus in tissues and swab specimens from infected turkeys

J. C. Pedersen, D. A. Senne, B. Panigrahy, Donald Reynolds

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Conventional nested and TaqMan® reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of avian pneumovirus (APV) were evaluated and compared with virus isolation (VI) for sensitivity and specificity. Respiratory tissues and tracheal swabs were collected from experimentally inoculated turkeys between 1 and 21 days postinoculation (DPI) and tested by all detection methods. APV was detected by both RT-PCR procedures as early as 1 DPI and as late as 17 DPI, whereas virus was isolated only between 3 and 7 DPI. Pooled tracheal swab supernatant and dry swabs were excellent specimens for the detection of APV between 3 and 8 DPI. Turbinate and sinus specimens were the most productive samples over the entire collection period. Both RT-PCR assays were rapid and more sensitive than VI for the detection of APV in tissue and swab specimens from infected turkeys. RT-PCR allows for the rapid detection of APV from a variety of respiratory tissues as well as from dry swabs and tracheal swab supernatants. Antibody to APV was detected in 50% of the sampled APV-inoculated birds at 8 and 9 DPI by enzyme-linked immunosorbent assay (ELISA). Early seroconversion (8-10 DPI) allows antibody detection to be used as a screening tool for APV. Rapid and sensitive detection methods are needed for APV, a highly contagious disease affecting U.S. poultry.

Original languageEnglish (US)
Pages (from-to)581-592
Number of pages12
JournalAvian diseases
Volume45
Issue number3
DOIs
StatePublished - Jan 1 2001

Fingerprint

Metapneumovirus
Avian metapneumovirus
Reverse Transcription
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction
Viruses
viruses
tissues
Turbinates
Antibodies
seroconversion
assays
antibody detection
Poultry
sinuses
Birds
poultry
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
screening

Keywords

  • Avian pneumovirus
  • Fluorgenic probe
  • Reverse transcription-polymerase chain reaction
  • Swollen head syndrome
  • TaqMan®
  • Turkey rhinotracheitis

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Detection of avian pneumovirus in tissues and swab specimens from infected turkeys. / Pedersen, J. C.; Senne, D. A.; Panigrahy, B.; Reynolds, Donald.

In: Avian diseases, Vol. 45, No. 3, 01.01.2001, p. 581-592.

Research output: Contribution to journalArticle

Pedersen, J. C. ; Senne, D. A. ; Panigrahy, B. ; Reynolds, Donald. / Detection of avian pneumovirus in tissues and swab specimens from infected turkeys. In: Avian diseases. 2001 ; Vol. 45, No. 3. pp. 581-592.
@article{a40e0ed627e64b8fa38a08f381e5a97e,
title = "Detection of avian pneumovirus in tissues and swab specimens from infected turkeys",
abstract = "Conventional nested and TaqMan{\circledR} reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of avian pneumovirus (APV) were evaluated and compared with virus isolation (VI) for sensitivity and specificity. Respiratory tissues and tracheal swabs were collected from experimentally inoculated turkeys between 1 and 21 days postinoculation (DPI) and tested by all detection methods. APV was detected by both RT-PCR procedures as early as 1 DPI and as late as 17 DPI, whereas virus was isolated only between 3 and 7 DPI. Pooled tracheal swab supernatant and dry swabs were excellent specimens for the detection of APV between 3 and 8 DPI. Turbinate and sinus specimens were the most productive samples over the entire collection period. Both RT-PCR assays were rapid and more sensitive than VI for the detection of APV in tissue and swab specimens from infected turkeys. RT-PCR allows for the rapid detection of APV from a variety of respiratory tissues as well as from dry swabs and tracheal swab supernatants. Antibody to APV was detected in 50{\%} of the sampled APV-inoculated birds at 8 and 9 DPI by enzyme-linked immunosorbent assay (ELISA). Early seroconversion (8-10 DPI) allows antibody detection to be used as a screening tool for APV. Rapid and sensitive detection methods are needed for APV, a highly contagious disease affecting U.S. poultry.",
keywords = "Avian pneumovirus, Fluorgenic probe, Reverse transcription-polymerase chain reaction, Swollen head syndrome, TaqMan{\circledR}, Turkey rhinotracheitis",
author = "Pedersen, {J. C.} and Senne, {D. A.} and B. Panigrahy and Donald Reynolds",
year = "2001",
month = "1",
day = "1",
doi = "10.2307/1592898",
language = "English (US)",
volume = "45",
pages = "581--592",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "3",

}

TY - JOUR

T1 - Detection of avian pneumovirus in tissues and swab specimens from infected turkeys

AU - Pedersen, J. C.

AU - Senne, D. A.

AU - Panigrahy, B.

AU - Reynolds, Donald

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Conventional nested and TaqMan® reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of avian pneumovirus (APV) were evaluated and compared with virus isolation (VI) for sensitivity and specificity. Respiratory tissues and tracheal swabs were collected from experimentally inoculated turkeys between 1 and 21 days postinoculation (DPI) and tested by all detection methods. APV was detected by both RT-PCR procedures as early as 1 DPI and as late as 17 DPI, whereas virus was isolated only between 3 and 7 DPI. Pooled tracheal swab supernatant and dry swabs were excellent specimens for the detection of APV between 3 and 8 DPI. Turbinate and sinus specimens were the most productive samples over the entire collection period. Both RT-PCR assays were rapid and more sensitive than VI for the detection of APV in tissue and swab specimens from infected turkeys. RT-PCR allows for the rapid detection of APV from a variety of respiratory tissues as well as from dry swabs and tracheal swab supernatants. Antibody to APV was detected in 50% of the sampled APV-inoculated birds at 8 and 9 DPI by enzyme-linked immunosorbent assay (ELISA). Early seroconversion (8-10 DPI) allows antibody detection to be used as a screening tool for APV. Rapid and sensitive detection methods are needed for APV, a highly contagious disease affecting U.S. poultry.

AB - Conventional nested and TaqMan® reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of avian pneumovirus (APV) were evaluated and compared with virus isolation (VI) for sensitivity and specificity. Respiratory tissues and tracheal swabs were collected from experimentally inoculated turkeys between 1 and 21 days postinoculation (DPI) and tested by all detection methods. APV was detected by both RT-PCR procedures as early as 1 DPI and as late as 17 DPI, whereas virus was isolated only between 3 and 7 DPI. Pooled tracheal swab supernatant and dry swabs were excellent specimens for the detection of APV between 3 and 8 DPI. Turbinate and sinus specimens were the most productive samples over the entire collection period. Both RT-PCR assays were rapid and more sensitive than VI for the detection of APV in tissue and swab specimens from infected turkeys. RT-PCR allows for the rapid detection of APV from a variety of respiratory tissues as well as from dry swabs and tracheal swab supernatants. Antibody to APV was detected in 50% of the sampled APV-inoculated birds at 8 and 9 DPI by enzyme-linked immunosorbent assay (ELISA). Early seroconversion (8-10 DPI) allows antibody detection to be used as a screening tool for APV. Rapid and sensitive detection methods are needed for APV, a highly contagious disease affecting U.S. poultry.

KW - Avian pneumovirus

KW - Fluorgenic probe

KW - Reverse transcription-polymerase chain reaction

KW - Swollen head syndrome

KW - TaqMan®

KW - Turkey rhinotracheitis

UR - http://www.scopus.com/inward/record.url?scp=0034813485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034813485&partnerID=8YFLogxK

U2 - 10.2307/1592898

DO - 10.2307/1592898

M3 - Article

VL - 45

SP - 581

EP - 592

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 3

ER -