Detection of autoreactive myelin proteolipid protein 139-151-specific T cells by using MHC II (IAs) tetramers

Jayagopala Reddy, Estelle Bettelli, Lindsay Nicholson, Hanspeter Waldner, Mei Huei Jang, Kai W. Wucherpfennig, Vijay K. Kuchroo

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62 Scopus citations

Abstract

Detection of autoreactive T cells using MHC II tetramers is difficult because of the low affinity of their TCR. We have generated a class II tetramer using the IAs class II molecule combined with an autoantigenic peptide from myelin proteolipid protein (PLP; PLP139-151) and used it to analyze myelin PLP139-151-reactive T cells. Using monomers and multimerized complexes labeled with PE, we confirmed the specificity of the reagent by bioassay and flow cytometry. The IAs tetramers stimulated and stained the PLP139-151-specific 5B6 TCR transgenic T cells and a polyclonal cell line specific for PLP139-151, but not a control T cell line specific for PLP178-191. We used this reagent to optimize conditions to detect low affinity autoreactive T cells. We found that high pH (∼8.0) and neuraminidase treatment enhances the staining capacity of PLP139-151 tetramer without compromising specificity. Furthermore, we found that induction of calcium fluxing by tetramers in T cells may be used as a sensitive measure to detect autoreactive T cells with a low affinity. Taken together, the data show that the tetrameric reagent binds and stimulates PLP139-151-reactive T cells with specificity. This tetrameric reagent will be useful in studying the evolution of PLP139-151-specific repertoire in naive mice and its expansion during the autoimmune disease experimental autoimmune encephalomyelitis.

Original languageEnglish (US)
Pages (from-to)870-877
Number of pages8
JournalJournal of Immunology
Volume170
Issue number2
DOIs
StatePublished - Jan 15 2003

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Reddy, J., Bettelli, E., Nicholson, L., Waldner, H., Jang, M. H., Wucherpfennig, K. W., & Kuchroo, V. K. (2003). Detection of autoreactive myelin proteolipid protein 139-151-specific T cells by using MHC II (IAs) tetramers. Journal of Immunology, 170(2), 870-877. https://doi.org/10.4049/jimmunol.170.2.870