Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome

Igor Morozov, Theerapol Sirinarumitr, Steven D. Sorden, Patrick G. Halbur, Marsha K. Morgan, Kyoung Jin Yoon, Prem S. Paul

Research output: Contribution to journalArticle

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Abstract

Swine infectious agents, especially viruses, are potential public health risks associated with the use of pig organs for xenotransplantation in humans. Therefore, there is a need for better characterization of swine viruses and for the development of diagnostic tests for their detection. We report here isolation of a novel strain of porcine circovirus (PCV) from pigs with postweaning multisystemic wasting syndrome (PMWS). Affected pigs exhibited severe interstitial pneumonia and lymphoid depletion. The complete nucleotide sequence (1,768 nucleotides) of the genome of the PCV isolate was determined and compared with the sequence of the PCV strain isolated from PK- 15 cells. Sequence comparison revealed significant differences between the two PCV strains, with an overall DNA homology of 76%. Two major open reading frames (ORFs) were identified. ORF1 was more conserved between the two strains, with 83% nucleotide homology and 86% amino acid homology. ORF2 was more variable, with nucleotide homology of 67% and amino acid homology of 65%. PCR and in situ hybridization demonstrated abundant viral DNA in various organs of pigs with PMWS. In situ hybridization demonstrated that this strain of PCV targets multiple organs and infects macrophages, lymphocytes, endothelial cells, and epithelial cells.

Original languageEnglish (US)
Pages (from-to)2535-2541
Number of pages7
JournalJournal of clinical microbiology
Volume36
Issue number9
StatePublished - Sep 1 1998

Fingerprint

Circovirus
Wasting Syndrome
Swine
Nucleotides
In Situ Hybridization
Viruses
Amino Acids
Heterologous Transplantation
Viral DNA
Routine Diagnostic Tests
Open Reading Frames
Endothelial Cells
Public Health
Epithelial Cells
Macrophages
Genome
Lymphocytes
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Morozov, I., Sirinarumitr, T., Sorden, S. D., Halbur, P. G., Morgan, M. K., Yoon, K. J., & Paul, P. S. (1998). Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. Journal of clinical microbiology, 36(9), 2535-2541.

Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. / Morozov, Igor; Sirinarumitr, Theerapol; Sorden, Steven D.; Halbur, Patrick G.; Morgan, Marsha K.; Yoon, Kyoung Jin; Paul, Prem S.

In: Journal of clinical microbiology, Vol. 36, No. 9, 01.09.1998, p. 2535-2541.

Research output: Contribution to journalArticle

Morozov, I, Sirinarumitr, T, Sorden, SD, Halbur, PG, Morgan, MK, Yoon, KJ & Paul, PS 1998, 'Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome', Journal of clinical microbiology, vol. 36, no. 9, pp. 2535-2541.
Morozov I, Sirinarumitr T, Sorden SD, Halbur PG, Morgan MK, Yoon KJ et al. Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. Journal of clinical microbiology. 1998 Sep 1;36(9):2535-2541.
Morozov, Igor ; Sirinarumitr, Theerapol ; Sorden, Steven D. ; Halbur, Patrick G. ; Morgan, Marsha K. ; Yoon, Kyoung Jin ; Paul, Prem S. / Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. In: Journal of clinical microbiology. 1998 ; Vol. 36, No. 9. pp. 2535-2541.
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