Detection and typing of human papillomavirus in archival cervical cancer specimens by DNA amplification with consensus primers

Robert M. Resnick, Marion T.E. Cornelissen, Deann K. Wright, Greg H. Eichinger, Howard S. Fox, Jan Ter Schegget, M. Michele Manos

Research output: Contribution to journalArticle

431 Citations (Scopus)

Abstract

We developed a polymerase chain reaction DNA amplification system using two distinct consensus oligonucleotide primer sets for the improved detection and typing of a broad spectrum of human genital papillomavirus (HPV) sequences, including those of novel viruses. The system incorporates one primer set designed to amplify a highly conserved L1 domain and a second primer set designed to amplify a domain within the E6 gene. We used this system to analyze 48 fixed, paraffin-embedded tissue sections (41 specimens from 33 cervical carcinomas, four normal cervical tissues, and several control tissues) for the presence of HPV DNA. HPV sequences were detected in all carcinoma samples and none of the control samples. Hybridization analyses showed that the results obtained with the two amplification schemes concurred completely. This approach allowed rapid confirmation of typing results and may improve the likelihood of detecting a wide variety of HPV sequences, including those of novel HPVs. [J Natl Cancer Inst 82:1477-1484, 1990].

Original languageEnglish (US)
Pages (from-to)1477-1484
Number of pages8
JournalJournal of the National Cancer Institute
Volume82
Issue number18
DOIs
StatePublished - Sep 19 1990

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Uterine Cervical Neoplasms
DNA
Carcinoma
DNA Primers
Paraffin
Viruses
Polymerase Chain Reaction
Genes
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Detection and typing of human papillomavirus in archival cervical cancer specimens by DNA amplification with consensus primers. / Resnick, Robert M.; Cornelissen, Marion T.E.; Wright, Deann K.; Eichinger, Greg H.; Fox, Howard S.; Schegget, Jan Ter; Manos, M. Michele.

In: Journal of the National Cancer Institute, Vol. 82, No. 18, 19.09.1990, p. 1477-1484.

Research output: Contribution to journalArticle

Resnick, Robert M. ; Cornelissen, Marion T.E. ; Wright, Deann K. ; Eichinger, Greg H. ; Fox, Howard S. ; Schegget, Jan Ter ; Manos, M. Michele. / Detection and typing of human papillomavirus in archival cervical cancer specimens by DNA amplification with consensus primers. In: Journal of the National Cancer Institute. 1990 ; Vol. 82, No. 18. pp. 1477-1484.
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