Detection and quantification of poliovirus infection using FTIR spectroscopy and cell culture

Felipe T. Lee-Montiel, Kelly A. Reynolds, Mark Riley

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, homeland security and environmental protection. Current virus detection methods, especially assessing viral infectivity, are complex and time-consuming, making point-of-care detection a challenge. Faster, more sensitive, highly specific methods are needed to quantify potentially hazardous viral pathogens and to determine if suspected materials contain viable viral particles. Fourier transform infrared (FTIR) spectroscopy combined with cellular-based sensing, may offer a precise way to detect specific viruses. This approach utilizes infrared light to monitor changes in molecular components of cells by tracking changes in absorbance patterns produced following virus infection. In this work poliovirus (PV1) was used to evaluate the utility of FTIR spectroscopy with cell culture for rapid detection of infective virus particles.Results: Buffalo green monkey kidney (BGMK) cells infected with different virus titers were studied at 1 - 12 hours post-infection (h.p.i.). A partial least squares (PLS) regression method was used to analyze and model cellular responses to different infection titers and times post-infection. The model performs best at 8 h.p.i., resulting in an estimated root mean square error of cross validation (RMSECV) of 17 plaque forming units (PFU)/ml when using low titers of infection of 10 and 100 PFU/ml. Higher titers, from 10 3 to 10 6 PFU/ml, could also be reliably detected.Conclusions: This approach to poliovirus detection and quantification using FTIR spectroscopy and cell culture could potentially be extended to compare biochemical cell responses to infection with different viruses. This virus detection method could feasibly be adapted to an automated scheme for use in areas such as water safety monitoring and medical diagnostics.

Original languageEnglish (US)
Article number16
JournalJournal of Biological Engineering
Volume5
DOIs
StatePublished - Dec 5 2011

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Poliovirus
Fourier Transform Infrared Spectroscopy
Viruses
Cell culture
Fourier transform infrared spectroscopy
Cell Culture Techniques
Infection
Virion
Point-of-Care Systems
Cell Tracking
Cercopithecus aethiops
Water
Buffaloes
Conservation of Natural Resources
Public risks
Virus Diseases
Least-Squares Analysis
Viral Load
Communicable Diseases
National security

Keywords

  • Buffalo green monkey kidney (bgmk) cells
  • Cell culture
  • Enterovirus
  • Fourier transform infrared (ftir) spectroscopy
  • Mid-infrared
  • Partial least squares
  • Poliovirus (pv1)
  • Virus detection
  • Zinc selenide (znse)

ASJC Scopus subject areas

  • Environmental Engineering
  • Biomedical Engineering
  • Molecular Biology
  • Cell Biology

Cite this

Detection and quantification of poliovirus infection using FTIR spectroscopy and cell culture. / Lee-Montiel, Felipe T.; Reynolds, Kelly A.; Riley, Mark.

In: Journal of Biological Engineering, Vol. 5, 16, 05.12.2011.

Research output: Contribution to journalArticle

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abstract = "Background: In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, homeland security and environmental protection. Current virus detection methods, especially assessing viral infectivity, are complex and time-consuming, making point-of-care detection a challenge. Faster, more sensitive, highly specific methods are needed to quantify potentially hazardous viral pathogens and to determine if suspected materials contain viable viral particles. Fourier transform infrared (FTIR) spectroscopy combined with cellular-based sensing, may offer a precise way to detect specific viruses. This approach utilizes infrared light to monitor changes in molecular components of cells by tracking changes in absorbance patterns produced following virus infection. In this work poliovirus (PV1) was used to evaluate the utility of FTIR spectroscopy with cell culture for rapid detection of infective virus particles.Results: Buffalo green monkey kidney (BGMK) cells infected with different virus titers were studied at 1 - 12 hours post-infection (h.p.i.). A partial least squares (PLS) regression method was used to analyze and model cellular responses to different infection titers and times post-infection. The model performs best at 8 h.p.i., resulting in an estimated root mean square error of cross validation (RMSECV) of 17 plaque forming units (PFU)/ml when using low titers of infection of 10 and 100 PFU/ml. Higher titers, from 10 3 to 10 6 PFU/ml, could also be reliably detected.Conclusions: This approach to poliovirus detection and quantification using FTIR spectroscopy and cell culture could potentially be extended to compare biochemical cell responses to infection with different viruses. This virus detection method could feasibly be adapted to an automated scheme for use in areas such as water safety monitoring and medical diagnostics.",
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