Detection and identification of variola virus DNA in aerosol collection filters and/or liquids

Ted Hadfield, Victoria Olson, Douglas Anders, Linda Beck, Donna Boston, Kenneth Damer, Inger Damon, Matthew Davenport, Christina Egan, Steven Heye Hinrichs, Penny Hitchcock, Anthony Hitchins, John Huggins, Mohamed Ibrahim, Paul Jackson, Liz Kerrigan, Karen Kesterson, Katalin Kiss, Angelo Madonna, Hermann MeyerDenise Pettit, Frank Schaefer, Mark Scheckelhoff, Elizabeth Vitalis, Scott Coates

Research output: Contribution to journalArticle

Abstract

The method of detection and identification of Variola virus DNA in aerosol collection filters and/or liquids is discussed. The analytical technique used is polymerase chain reaction (PCR) methods. The predetermined minimum level of an analyte, as specified by an expert committee, which must be detected by the candidate method at a specified probability of detection (POD) is necessary. The inclusivity panel shall include sequences from at least two representative strains from each major clade of Variola virus Any other strain with differences in the assay primer and/or probe target sequences based on bioinformatic analysis. Inclusion of all environmental panel organisms is not a requirement if a method developer provides appropriate justification that the intended use of the assay permits the exclusion of specific panel organisms. Justification for exclusion of any environmental panel organism(s) must be documented and submitted.

Original languageEnglish (US)
Pages (from-to)1046-1049
Number of pages4
JournalJournal of AOAC International
Volume98
Issue number4
DOIs
StatePublished - Jul 1 2015

Fingerprint

Variola virus
aerosols
Aerosols
Viruses
Assays
virus
aerosol
filter
DNA
liquid
liquids
Polymerase chain reaction
Liquids
Bioinformatics
organisms
assay
bioinformatics
assays
Computational Biology
methodology

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Environmental Chemistry
  • Agronomy and Crop Science
  • Pharmacology

Cite this

Hadfield, T., Olson, V., Anders, D., Beck, L., Boston, D., Damer, K., ... Coates, S. (2015). Detection and identification of variola virus DNA in aerosol collection filters and/or liquids. Journal of AOAC International, 98(4), 1046-1049. https://doi.org/10.5740/jaoac.int.SMPR2014.006

Detection and identification of variola virus DNA in aerosol collection filters and/or liquids. / Hadfield, Ted; Olson, Victoria; Anders, Douglas; Beck, Linda; Boston, Donna; Damer, Kenneth; Damon, Inger; Davenport, Matthew; Egan, Christina; Hinrichs, Steven Heye; Hitchcock, Penny; Hitchins, Anthony; Huggins, John; Ibrahim, Mohamed; Jackson, Paul; Kerrigan, Liz; Kesterson, Karen; Kiss, Katalin; Madonna, Angelo; Meyer, Hermann; Pettit, Denise; Schaefer, Frank; Scheckelhoff, Mark; Vitalis, Elizabeth; Coates, Scott.

In: Journal of AOAC International, Vol. 98, No. 4, 01.07.2015, p. 1046-1049.

Research output: Contribution to journalArticle

Hadfield, T, Olson, V, Anders, D, Beck, L, Boston, D, Damer, K, Damon, I, Davenport, M, Egan, C, Hinrichs, SH, Hitchcock, P, Hitchins, A, Huggins, J, Ibrahim, M, Jackson, P, Kerrigan, L, Kesterson, K, Kiss, K, Madonna, A, Meyer, H, Pettit, D, Schaefer, F, Scheckelhoff, M, Vitalis, E & Coates, S 2015, 'Detection and identification of variola virus DNA in aerosol collection filters and/or liquids', Journal of AOAC International, vol. 98, no. 4, pp. 1046-1049. https://doi.org/10.5740/jaoac.int.SMPR2014.006
Hadfield, Ted ; Olson, Victoria ; Anders, Douglas ; Beck, Linda ; Boston, Donna ; Damer, Kenneth ; Damon, Inger ; Davenport, Matthew ; Egan, Christina ; Hinrichs, Steven Heye ; Hitchcock, Penny ; Hitchins, Anthony ; Huggins, John ; Ibrahim, Mohamed ; Jackson, Paul ; Kerrigan, Liz ; Kesterson, Karen ; Kiss, Katalin ; Madonna, Angelo ; Meyer, Hermann ; Pettit, Denise ; Schaefer, Frank ; Scheckelhoff, Mark ; Vitalis, Elizabeth ; Coates, Scott. / Detection and identification of variola virus DNA in aerosol collection filters and/or liquids. In: Journal of AOAC International. 2015 ; Vol. 98, No. 4. pp. 1046-1049.
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