Detecting Microbial Dysbiosis Associated with Pediatric Crohn Disease Despite the High Variability of the Gut Microbiota

Feng Wang, Jess L. Kaplan, Benjamin D. Gold, Manoj K. Bhasin, Naomi L. Ward, Richard Kellermayer, Barbara S. Kirschner, Melvin B. Heyman, Scot E. Dowd, Stephen B. Cox, Haluk Dogan, Blaire Steven, George D. Ferry, Stanley A. Cohen, Robert N. Baldassano, Christopher J. Moran, Elizabeth A. Garnett, Lauren Drake, Hasan H Otu, Leonid A. Mirny & 3 others Towia A. Libermann, Harland S. Winter, Kirill S. Korolev

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The relationship between the host and its microbiota is challenging to understand because both microbial communities and their environments are highly variable. We have developed a set of techniques based on population dynamics and information theory to address this challenge. These methods identify additional bacterial taxa associated with pediatric Crohn disease and can detect significant changes in microbial communities with fewer samples than previous statistical approaches required. We have also substantially improved the accuracy of the diagnosis based on the microbiota from stool samples, and we found that the ecological niche of a microbe predicts its role in Crohn disease. Bacteria typically residing in the lumen of healthy individuals decrease in disease, whereas bacteria typically residing on the mucosa of healthy individuals increase in disease. Our results also show that the associations with Crohn disease are evolutionarily conserved and provide a mutual information-based method to depict dysbiosis.

Original languageEnglish (US)
Pages (from-to)945-955
Number of pages11
JournalCell Reports
Volume14
Issue number4
DOIs
StatePublished - Feb 2 2016

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Dysbiosis
Pediatrics
Microbiota
Population Dynamics
Crohn Disease
Information Theory
Bacteria
Mucous Membrane
Population dynamics
Information theory
Gastrointestinal Microbiome
Pediatric Crohn's disease

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wang, F., Kaplan, J. L., Gold, B. D., Bhasin, M. K., Ward, N. L., Kellermayer, R., ... Korolev, K. S. (2016). Detecting Microbial Dysbiosis Associated with Pediatric Crohn Disease Despite the High Variability of the Gut Microbiota. Cell Reports, 14(4), 945-955. https://doi.org/10.1016/j.celrep.2015.12.088

Detecting Microbial Dysbiosis Associated with Pediatric Crohn Disease Despite the High Variability of the Gut Microbiota. / Wang, Feng; Kaplan, Jess L.; Gold, Benjamin D.; Bhasin, Manoj K.; Ward, Naomi L.; Kellermayer, Richard; Kirschner, Barbara S.; Heyman, Melvin B.; Dowd, Scot E.; Cox, Stephen B.; Dogan, Haluk; Steven, Blaire; Ferry, George D.; Cohen, Stanley A.; Baldassano, Robert N.; Moran, Christopher J.; Garnett, Elizabeth A.; Drake, Lauren; Otu, Hasan H; Mirny, Leonid A.; Libermann, Towia A.; Winter, Harland S.; Korolev, Kirill S.

In: Cell Reports, Vol. 14, No. 4, 02.02.2016, p. 945-955.

Research output: Contribution to journalArticle

Wang, F, Kaplan, JL, Gold, BD, Bhasin, MK, Ward, NL, Kellermayer, R, Kirschner, BS, Heyman, MB, Dowd, SE, Cox, SB, Dogan, H, Steven, B, Ferry, GD, Cohen, SA, Baldassano, RN, Moran, CJ, Garnett, EA, Drake, L, Otu, HH, Mirny, LA, Libermann, TA, Winter, HS & Korolev, KS 2016, 'Detecting Microbial Dysbiosis Associated with Pediatric Crohn Disease Despite the High Variability of the Gut Microbiota', Cell Reports, vol. 14, no. 4, pp. 945-955. https://doi.org/10.1016/j.celrep.2015.12.088
Wang, Feng ; Kaplan, Jess L. ; Gold, Benjamin D. ; Bhasin, Manoj K. ; Ward, Naomi L. ; Kellermayer, Richard ; Kirschner, Barbara S. ; Heyman, Melvin B. ; Dowd, Scot E. ; Cox, Stephen B. ; Dogan, Haluk ; Steven, Blaire ; Ferry, George D. ; Cohen, Stanley A. ; Baldassano, Robert N. ; Moran, Christopher J. ; Garnett, Elizabeth A. ; Drake, Lauren ; Otu, Hasan H ; Mirny, Leonid A. ; Libermann, Towia A. ; Winter, Harland S. ; Korolev, Kirill S. / Detecting Microbial Dysbiosis Associated with Pediatric Crohn Disease Despite the High Variability of the Gut Microbiota. In: Cell Reports. 2016 ; Vol. 14, No. 4. pp. 945-955.
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