Desorption ionization combined with tandem mass spectrometry: Advantages for investigating complex lipids, disaccharides and organometallic complexes

Ronald Cerny, Kenneth B. Tomer, Michael L. Gross

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Combining desorption ionization with tandem mass spectrometry overcomes the disadvantage of limited fragmentation accompanying desorption and permits mixtures of closely related substances to be investigated directly. These features of the combination are illustrated by completing the structure‐proof of a minor component of an ornithine‐containing lipid mixture isolated from Thiobacillus thiooxidans. The minor component is a homolog of the major constituent and differs from the principal component owing to the presence of a double bond in lieu of a cyclopropyl ring in one of the constituent fatty acids. Another feature of the combined method is the potentially complementary nature of collision‐activated dissociation spectra of protonated and cationized biomolecules. This is illustrated by the differences in the collision‐activated dissociations of the [M + Na]+ of sucrose, desorbed by field desorption, and [M + H]+, desorbed by fast atom bombardment. A third illustration is the application of field desorption and tandem mass spectrometry to an organometallic compound. The combined approach allows the ligands to be identified and the relative ligand binding energies to be approximated.

Original languageEnglish (US)
Pages (from-to)655-660
Number of pages6
JournalOrganic Mass Spectrometry
Volume21
Issue number10
DOIs
StatePublished - Oct 1986

Fingerprint

Disaccharides
Organometallics
Tandem Mass Spectrometry
Lipids
Ionization
Mass spectrometry
lipids
Desorption
Acidithiobacillus thiooxidans
mass spectroscopy
desorption
Organometallic Compounds
Ligands
ionization
Sucrose
Fatty Acids
dissociation
organometallic compounds
ligands
sucrose

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Instrumentation

Cite this

Desorption ionization combined with tandem mass spectrometry : Advantages for investigating complex lipids, disaccharides and organometallic complexes. / Cerny, Ronald; Tomer, Kenneth B.; Gross, Michael L.

In: Organic Mass Spectrometry, Vol. 21, No. 10, 10.1986, p. 655-660.

Research output: Contribution to journalArticle

@article{133caf27f07142b1a31236a6c7545ef8,
title = "Desorption ionization combined with tandem mass spectrometry: Advantages for investigating complex lipids, disaccharides and organometallic complexes",
abstract = "Combining desorption ionization with tandem mass spectrometry overcomes the disadvantage of limited fragmentation accompanying desorption and permits mixtures of closely related substances to be investigated directly. These features of the combination are illustrated by completing the structure‐proof of a minor component of an ornithine‐containing lipid mixture isolated from Thiobacillus thiooxidans. The minor component is a homolog of the major constituent and differs from the principal component owing to the presence of a double bond in lieu of a cyclopropyl ring in one of the constituent fatty acids. Another feature of the combined method is the potentially complementary nature of collision‐activated dissociation spectra of protonated and cationized biomolecules. This is illustrated by the differences in the collision‐activated dissociations of the [M + Na]+ of sucrose, desorbed by field desorption, and [M + H]+, desorbed by fast atom bombardment. A third illustration is the application of field desorption and tandem mass spectrometry to an organometallic compound. The combined approach allows the ligands to be identified and the relative ligand binding energies to be approximated.",
author = "Ronald Cerny and Tomer, {Kenneth B.} and Gross, {Michael L.}",
year = "1986",
month = "10",
doi = "10.1002/oms.1210211009",
language = "English (US)",
volume = "21",
pages = "655--660",
journal = "Organic Mass Spectrometry",
issn = "0030-493X",
publisher = "John Wiley and Sons Ltd",
number = "10",

}

TY - JOUR

T1 - Desorption ionization combined with tandem mass spectrometry

T2 - Advantages for investigating complex lipids, disaccharides and organometallic complexes

AU - Cerny, Ronald

AU - Tomer, Kenneth B.

AU - Gross, Michael L.

PY - 1986/10

Y1 - 1986/10

N2 - Combining desorption ionization with tandem mass spectrometry overcomes the disadvantage of limited fragmentation accompanying desorption and permits mixtures of closely related substances to be investigated directly. These features of the combination are illustrated by completing the structure‐proof of a minor component of an ornithine‐containing lipid mixture isolated from Thiobacillus thiooxidans. The minor component is a homolog of the major constituent and differs from the principal component owing to the presence of a double bond in lieu of a cyclopropyl ring in one of the constituent fatty acids. Another feature of the combined method is the potentially complementary nature of collision‐activated dissociation spectra of protonated and cationized biomolecules. This is illustrated by the differences in the collision‐activated dissociations of the [M + Na]+ of sucrose, desorbed by field desorption, and [M + H]+, desorbed by fast atom bombardment. A third illustration is the application of field desorption and tandem mass spectrometry to an organometallic compound. The combined approach allows the ligands to be identified and the relative ligand binding energies to be approximated.

AB - Combining desorption ionization with tandem mass spectrometry overcomes the disadvantage of limited fragmentation accompanying desorption and permits mixtures of closely related substances to be investigated directly. These features of the combination are illustrated by completing the structure‐proof of a minor component of an ornithine‐containing lipid mixture isolated from Thiobacillus thiooxidans. The minor component is a homolog of the major constituent and differs from the principal component owing to the presence of a double bond in lieu of a cyclopropyl ring in one of the constituent fatty acids. Another feature of the combined method is the potentially complementary nature of collision‐activated dissociation spectra of protonated and cationized biomolecules. This is illustrated by the differences in the collision‐activated dissociations of the [M + Na]+ of sucrose, desorbed by field desorption, and [M + H]+, desorbed by fast atom bombardment. A third illustration is the application of field desorption and tandem mass spectrometry to an organometallic compound. The combined approach allows the ligands to be identified and the relative ligand binding energies to be approximated.

UR - http://www.scopus.com/inward/record.url?scp=84989092818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989092818&partnerID=8YFLogxK

U2 - 10.1002/oms.1210211009

DO - 10.1002/oms.1210211009

M3 - Article

AN - SCOPUS:84989092818

VL - 21

SP - 655

EP - 660

JO - Organic Mass Spectrometry

JF - Organic Mass Spectrometry

SN - 0030-493X

IS - 10

ER -