Desmosome dynamics in migrating epithelial cells requires the actin cytoskeleton

Brett J. Roberts, Anjeza Pashaj, Keith R Johnson, James K Wahl

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Re-modeling of epithelial tissues requires that the cells in the tissue rearrange their adhesive contacts in order to allow cells to migrate relative to neighboring cells. Desmosomes are prominent adhesive structures found in a variety of epithelial tissues that are believed to inhibit cell migration and invasion. Mechanisms regulating desmosome assembly and stability in migrating cells are largely unknown. In this study we established a cell culture model to examine the fate of desmosomal components during scratch wound migration. Desmosomes are rapidly assembled between epithelial cells at the lateral edges of migrating cells and structures are transported in a retrograde fashion while the structures become larger and mature. Desmosome assembly and dynamics in this system are dependent on the actin cytoskeleton prior to being associated with the keratin intermediate filament cytoskeleton. These studies extend our understanding of desmosome assembly and provide a system to examine desmosome assembly and dynamics during epithelial cell migration.

Original languageEnglish (US)
Pages (from-to)2814-2822
Number of pages9
JournalExperimental Cell Research
Volume317
Issue number20
DOIs
StatePublished - Jan 1 2011

Fingerprint

Desmosomes
Actin Cytoskeleton
Epithelial Cells
Adhesives
Cell Movement
Epithelium
Intermediate Filaments
Keratins
Cytoskeleton
Cell Culture Techniques
Wounds and Injuries

Keywords

  • Actin
  • Desmocollin
  • Desmosome
  • Live cell microscopy
  • Motility
  • Plakophilin-3

ASJC Scopus subject areas

  • Cell Biology

Cite this

Desmosome dynamics in migrating epithelial cells requires the actin cytoskeleton. / Roberts, Brett J.; Pashaj, Anjeza; Johnson, Keith R; Wahl, James K.

In: Experimental Cell Research, Vol. 317, No. 20, 01.01.2011, p. 2814-2822.

Research output: Contribution to journalArticle

@article{3270cc6d3e1b4c7c802e36419e019d95,
title = "Desmosome dynamics in migrating epithelial cells requires the actin cytoskeleton",
abstract = "Re-modeling of epithelial tissues requires that the cells in the tissue rearrange their adhesive contacts in order to allow cells to migrate relative to neighboring cells. Desmosomes are prominent adhesive structures found in a variety of epithelial tissues that are believed to inhibit cell migration and invasion. Mechanisms regulating desmosome assembly and stability in migrating cells are largely unknown. In this study we established a cell culture model to examine the fate of desmosomal components during scratch wound migration. Desmosomes are rapidly assembled between epithelial cells at the lateral edges of migrating cells and structures are transported in a retrograde fashion while the structures become larger and mature. Desmosome assembly and dynamics in this system are dependent on the actin cytoskeleton prior to being associated with the keratin intermediate filament cytoskeleton. These studies extend our understanding of desmosome assembly and provide a system to examine desmosome assembly and dynamics during epithelial cell migration.",
keywords = "Actin, Desmocollin, Desmosome, Live cell microscopy, Motility, Plakophilin-3",
author = "Roberts, {Brett J.} and Anjeza Pashaj and Johnson, {Keith R} and Wahl, {James K}",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/j.yexcr.2011.09.003",
language = "English (US)",
volume = "317",
pages = "2814--2822",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "20",

}

TY - JOUR

T1 - Desmosome dynamics in migrating epithelial cells requires the actin cytoskeleton

AU - Roberts, Brett J.

AU - Pashaj, Anjeza

AU - Johnson, Keith R

AU - Wahl, James K

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Re-modeling of epithelial tissues requires that the cells in the tissue rearrange their adhesive contacts in order to allow cells to migrate relative to neighboring cells. Desmosomes are prominent adhesive structures found in a variety of epithelial tissues that are believed to inhibit cell migration and invasion. Mechanisms regulating desmosome assembly and stability in migrating cells are largely unknown. In this study we established a cell culture model to examine the fate of desmosomal components during scratch wound migration. Desmosomes are rapidly assembled between epithelial cells at the lateral edges of migrating cells and structures are transported in a retrograde fashion while the structures become larger and mature. Desmosome assembly and dynamics in this system are dependent on the actin cytoskeleton prior to being associated with the keratin intermediate filament cytoskeleton. These studies extend our understanding of desmosome assembly and provide a system to examine desmosome assembly and dynamics during epithelial cell migration.

AB - Re-modeling of epithelial tissues requires that the cells in the tissue rearrange their adhesive contacts in order to allow cells to migrate relative to neighboring cells. Desmosomes are prominent adhesive structures found in a variety of epithelial tissues that are believed to inhibit cell migration and invasion. Mechanisms regulating desmosome assembly and stability in migrating cells are largely unknown. In this study we established a cell culture model to examine the fate of desmosomal components during scratch wound migration. Desmosomes are rapidly assembled between epithelial cells at the lateral edges of migrating cells and structures are transported in a retrograde fashion while the structures become larger and mature. Desmosome assembly and dynamics in this system are dependent on the actin cytoskeleton prior to being associated with the keratin intermediate filament cytoskeleton. These studies extend our understanding of desmosome assembly and provide a system to examine desmosome assembly and dynamics during epithelial cell migration.

KW - Actin

KW - Desmocollin

KW - Desmosome

KW - Live cell microscopy

KW - Motility

KW - Plakophilin-3

UR - http://www.scopus.com/inward/record.url?scp=82555187993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82555187993&partnerID=8YFLogxK

U2 - 10.1016/j.yexcr.2011.09.003

DO - 10.1016/j.yexcr.2011.09.003

M3 - Article

VL - 317

SP - 2814

EP - 2822

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 20

ER -