Design and Fabrication of an MRI-Compatible, Autonomous Incubation System

Vahid Khalilzad-Sharghi, Huihui Xu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Tissue engineers have long sought access to an autonomous, imaging-compatible tissue incubation system that, with minimum operator handling, can provide real-time visualization and quantification of cells, tissue constructs, and organs. This type of screening system, capable of operating noninvasively to validate tissue, can overcome current limitations like temperature shock, unsustainable cellular environments, sample contamination, and handling/stress. However, this type of system has been a major challenge, until now. Here, we describe the design, fabrication, and characterization of an innovative, autonomous incubation system that is compatible with a 9.4 T magnetic resonance imaging (MRI) scanner. Termed the e-incubator (patent pending; application number: 13/953,984), this microcontroller-based system is integrated into an MRI scanner and noninvasively screens cells and tissue cultures in an environment where temperature, pH, and media/gas handling are regulated. The 4-week study discussed herein details the continuous operation of the e-incubator for a tissue-engineered osteogenic construct, validated by LIVE/DEAD® cell assays and histology. The evolving MR quantitative parameters of the osteogenic construct were used as biomarkers for bone tissue engineering and to further validate the quality of the product noninvasively before harvesting. Importantly, the e-incubator reliably facilitates culturing cells and tissue constructs to create engineered tissues and/or investigate disease therapies.

Original languageEnglish (US)
Pages (from-to)2406-2415
Number of pages10
JournalAnnals of biomedical engineering
Volume43
Issue number10
DOIs
StatePublished - Oct 22 2015

Fingerprint

Magnetic resonance
Tissue
Imaging techniques
Fabrication
Tissue culture
Histology
Biomarkers
Microcontrollers
Tissue engineering
Cell culture
Assays
Screening
Bone
Contamination
Visualization
Engineers
Temperature
Gases

Keywords

  • Bone tissue engineering microcontroller
  • MRI-compatible
  • Magnetic resonance imaging

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Design and Fabrication of an MRI-Compatible, Autonomous Incubation System. / Khalilzad-Sharghi, Vahid; Xu, Huihui.

In: Annals of biomedical engineering, Vol. 43, No. 10, 22.10.2015, p. 2406-2415.

Research output: Contribution to journalArticle

Khalilzad-Sharghi, Vahid ; Xu, Huihui. / Design and Fabrication of an MRI-Compatible, Autonomous Incubation System. In: Annals of biomedical engineering. 2015 ; Vol. 43, No. 10. pp. 2406-2415.
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