Derivation of oocytes from mouse embryonic stem cells

Karin Hübner, Guy Fuhrmann, Lane K. Christenson, James Kehler, Rolland Reinbold, Rabindranath De La Fuente, Jennifer Wood, Jerome F. Strauss, Michele Boiani, Hans R. Schöler

Research output: Contribution to journalArticle

860 Citations (Scopus)

Abstract

Continuation of mammalian species requires the formation and development of the sexually dimorphic germ cells. Cultured embryonic stem cells are generally considered pluripotent rather than totipotent because of the failure to detect germline cells under differentiating conditions. Here we show that mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop into blastocysts. Oogenesis in culture should contribute to various areas, including nuclear transfer and manipulation of the germ line, and advance studies on fertility treatment and germ and somatic cell interaction and differentiation.

Original languageEnglish (US)
Pages (from-to)1251-1256
Number of pages6
JournalScience
Volume300
Issue number5623
DOIs
StatePublished - May 23 2003

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Germ Cells
Oocytes
Oogonia
Oogenesis
Meiosis
Blastocyst
Embryonic Stem Cells
Cell Communication
Fertility
Cell Differentiation
Cell Culture Techniques
Mouse Embryonic Stem Cells

ASJC Scopus subject areas

  • General

Cite this

Hübner, K., Fuhrmann, G., Christenson, L. K., Kehler, J., Reinbold, R., De La Fuente, R., ... Schöler, H. R. (2003). Derivation of oocytes from mouse embryonic stem cells. Science, 300(5623), 1251-1256. https://doi.org/10.1126/science.1083452

Derivation of oocytes from mouse embryonic stem cells. / Hübner, Karin; Fuhrmann, Guy; Christenson, Lane K.; Kehler, James; Reinbold, Rolland; De La Fuente, Rabindranath; Wood, Jennifer; Strauss, Jerome F.; Boiani, Michele; Schöler, Hans R.

In: Science, Vol. 300, No. 5623, 23.05.2003, p. 1251-1256.

Research output: Contribution to journalArticle

Hübner, K, Fuhrmann, G, Christenson, LK, Kehler, J, Reinbold, R, De La Fuente, R, Wood, J, Strauss, JF, Boiani, M & Schöler, HR 2003, 'Derivation of oocytes from mouse embryonic stem cells', Science, vol. 300, no. 5623, pp. 1251-1256. https://doi.org/10.1126/science.1083452
Hübner K, Fuhrmann G, Christenson LK, Kehler J, Reinbold R, De La Fuente R et al. Derivation of oocytes from mouse embryonic stem cells. Science. 2003 May 23;300(5623):1251-1256. https://doi.org/10.1126/science.1083452
Hübner, Karin ; Fuhrmann, Guy ; Christenson, Lane K. ; Kehler, James ; Reinbold, Rolland ; De La Fuente, Rabindranath ; Wood, Jennifer ; Strauss, Jerome F. ; Boiani, Michele ; Schöler, Hans R. / Derivation of oocytes from mouse embryonic stem cells. In: Science. 2003 ; Vol. 300, No. 5623. pp. 1251-1256.
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