Deoxyribonucleic acid polymerase-α activity in hamster follicles during the estrous cycle

roles of follicle stimulating hormone and luteinizing hormone

Shyamal K Roy, Gilbert S. Greenwald

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the hamster, DNA polymerase-a (Pol-α) in follicles at stages 1-4 (1-4 layers granulosa cells and no theca) increased significantly during the proestrous (P) gonadotropin surges, remained high on estrus (E) and then declined to low levels by 09.00 h, proestrus (P). However, Pol-a in stages 5-8 (large preantral to small antral stages) remained steady throughout the cycle. Hypophysectomy on metestrus decreased Pol-a by 17 h which was reversed by 2.5 μg follicle stimulating hormone (FSH) but not by luteinizing hormone (LH) (2.5 jug) or human chorionic gonadotropin (hCG) (1 IU). Hypophysectomy on E resulted 72 h later in a fall in Pol-α in stages 1-6; FSH (2.5 fig) or LH (2 μg) restored enzyme activity within 5 h to stages 1-6 and 5-6, respectively. Thus, Pol-α in the smallest preantral follicles is induced by FSH; however, for large preantral and antral follicles, steady levels are maintained by tonic FSH and LH resulting in no apparent change in enzyme activity during active DNA synthesis.

Original languageEnglish (US)
Pages (from-to)157-166
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume61
Issue number2
DOIs
StatePublished - Jan 1 1989

Fingerprint

Estrous Cycle
Follicle Stimulating Hormone
Luteinizing Hormone
Cricetinae
Hypophysectomy
DNA
Enzyme activity
Metestrus
Ficus
Proestrus
Granulosa Cells
Estrus
Chorionic Gonadotropin
DNA-Directed DNA Polymerase
Enzymes
Gonadotropins
Antral

Keywords

  • (Hamster)
  • Gonadotropin
  • Polymerase-α
  • Preantral follicle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

@article{5b7c063795794aee9dbc067ea469df4c,
title = "Deoxyribonucleic acid polymerase-α activity in hamster follicles during the estrous cycle: roles of follicle stimulating hormone and luteinizing hormone",
abstract = "In the hamster, DNA polymerase-a (Pol-α) in follicles at stages 1-4 (1-4 layers granulosa cells and no theca) increased significantly during the proestrous (P) gonadotropin surges, remained high on estrus (E) and then declined to low levels by 09.00 h, proestrus (P). However, Pol-a in stages 5-8 (large preantral to small antral stages) remained steady throughout the cycle. Hypophysectomy on metestrus decreased Pol-a by 17 h which was reversed by 2.5 μg follicle stimulating hormone (FSH) but not by luteinizing hormone (LH) (2.5 jug) or human chorionic gonadotropin (hCG) (1 IU). Hypophysectomy on E resulted 72 h later in a fall in Pol-α in stages 1-6; FSH (2.5 fig) or LH (2 μg) restored enzyme activity within 5 h to stages 1-6 and 5-6, respectively. Thus, Pol-α in the smallest preantral follicles is induced by FSH; however, for large preantral and antral follicles, steady levels are maintained by tonic FSH and LH resulting in no apparent change in enzyme activity during active DNA synthesis.",
keywords = "(Hamster), Gonadotropin, Polymerase-α, Preantral follicle",
author = "Roy, {Shyamal K} and Greenwald, {Gilbert S.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0303-7207(89)90126-3",
language = "English (US)",
volume = "61",
pages = "157--166",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Deoxyribonucleic acid polymerase-α activity in hamster follicles during the estrous cycle

T2 - roles of follicle stimulating hormone and luteinizing hormone

AU - Roy, Shyamal K

AU - Greenwald, Gilbert S.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - In the hamster, DNA polymerase-a (Pol-α) in follicles at stages 1-4 (1-4 layers granulosa cells and no theca) increased significantly during the proestrous (P) gonadotropin surges, remained high on estrus (E) and then declined to low levels by 09.00 h, proestrus (P). However, Pol-a in stages 5-8 (large preantral to small antral stages) remained steady throughout the cycle. Hypophysectomy on metestrus decreased Pol-a by 17 h which was reversed by 2.5 μg follicle stimulating hormone (FSH) but not by luteinizing hormone (LH) (2.5 jug) or human chorionic gonadotropin (hCG) (1 IU). Hypophysectomy on E resulted 72 h later in a fall in Pol-α in stages 1-6; FSH (2.5 fig) or LH (2 μg) restored enzyme activity within 5 h to stages 1-6 and 5-6, respectively. Thus, Pol-α in the smallest preantral follicles is induced by FSH; however, for large preantral and antral follicles, steady levels are maintained by tonic FSH and LH resulting in no apparent change in enzyme activity during active DNA synthesis.

AB - In the hamster, DNA polymerase-a (Pol-α) in follicles at stages 1-4 (1-4 layers granulosa cells and no theca) increased significantly during the proestrous (P) gonadotropin surges, remained high on estrus (E) and then declined to low levels by 09.00 h, proestrus (P). However, Pol-a in stages 5-8 (large preantral to small antral stages) remained steady throughout the cycle. Hypophysectomy on metestrus decreased Pol-a by 17 h which was reversed by 2.5 μg follicle stimulating hormone (FSH) but not by luteinizing hormone (LH) (2.5 jug) or human chorionic gonadotropin (hCG) (1 IU). Hypophysectomy on E resulted 72 h later in a fall in Pol-α in stages 1-6; FSH (2.5 fig) or LH (2 μg) restored enzyme activity within 5 h to stages 1-6 and 5-6, respectively. Thus, Pol-α in the smallest preantral follicles is induced by FSH; however, for large preantral and antral follicles, steady levels are maintained by tonic FSH and LH resulting in no apparent change in enzyme activity during active DNA synthesis.

KW - (Hamster)

KW - Gonadotropin

KW - Polymerase-α

KW - Preantral follicle

UR - http://www.scopus.com/inward/record.url?scp=0024532623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024532623&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(89)90126-3

DO - 10.1016/0303-7207(89)90126-3

M3 - Article

VL - 61

SP - 157

EP - 166

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 2

ER -