Dentin sialoprotein is a novel substrate of matrix metalloproteinase 9 in vitro and in vivo

Guohua Yuan, Lei Chen, Junsheng Feng, Guobin Yang, Qingwen Ni, Xiaoping Xu, Chunyan Wan, Merry Lindsey, Kevin J. Donly, Mary MacDougall, Zhi Chen, Shuo Chen

Research output: Contribution to journalArticle

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Abstract

Dentin sialoprotein (DSP) is essential for dentinogenesis and processed into fragments in the odontoblast-like cells and the tooth compartments. Matrix metalloproteinase 9 (MMP9) is expressed in teeth from early embryonic to adult stage. Although MMP9 has been reported to be involved in some physiological and pathological conditions through processing substrates, its role in tooth development and whether DSP is a substrate of MMP9 remain unknown. In this study, the function of MMP9 in the tooth development was examined by observation of Mmp9 knockout (Mmp9-/-) mouse phenotype, and whether DSP is a substrate of MMP9 was explored by in vitro and in vivo experiments. The results showed that Mmp9-/- teeth displayed a phenotype similar to dentinogenesis imperfecta, including decreased dentin mineral density, abnormal dentin architecture, widened predentin and irregular predentin-dentin boundary. The distribution of MMP9 and DSP overlapped in the odontoblasts, the predentin, and the mineralized dentin, and MMP9 was able to specifically bind to DSP. MMP9 highly efficiently cleaved DSP into distinct fragments in vitro, and the deletion of Mmp9 caused improper processing of DSP in natural teeth. Therefore, our findings demonstrate that MMP9 is important for tooth development and DSP is a novel target of MMP9 during dentinogenesis.

Original languageEnglish (US)
Article number42449
JournalScientific reports
Volume7
DOIs
StatePublished - Feb 14 2017

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Matrix Metalloproteinase 9
Tooth
Dentin
Dentinogenesis
Odontoblasts
Dentinogenesis Imperfecta
In Vitro Techniques
dentin sialophosphoprotein
Phenotype
Knockout Mice
Minerals
Observation

ASJC Scopus subject areas

  • General

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Dentin sialoprotein is a novel substrate of matrix metalloproteinase 9 in vitro and in vivo. / Yuan, Guohua; Chen, Lei; Feng, Junsheng; Yang, Guobin; Ni, Qingwen; Xu, Xiaoping; Wan, Chunyan; Lindsey, Merry; Donly, Kevin J.; MacDougall, Mary; Chen, Zhi; Chen, Shuo.

In: Scientific reports, Vol. 7, 42449, 14.02.2017.

Research output: Contribution to journalArticle

Yuan, G, Chen, L, Feng, J, Yang, G, Ni, Q, Xu, X, Wan, C, Lindsey, M, Donly, KJ, MacDougall, M, Chen, Z & Chen, S 2017, 'Dentin sialoprotein is a novel substrate of matrix metalloproteinase 9 in vitro and in vivo', Scientific reports, vol. 7, 42449. https://doi.org/10.1038/srep42449
Yuan, Guohua ; Chen, Lei ; Feng, Junsheng ; Yang, Guobin ; Ni, Qingwen ; Xu, Xiaoping ; Wan, Chunyan ; Lindsey, Merry ; Donly, Kevin J. ; MacDougall, Mary ; Chen, Zhi ; Chen, Shuo. / Dentin sialoprotein is a novel substrate of matrix metalloproteinase 9 in vitro and in vivo. In: Scientific reports. 2017 ; Vol. 7.
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abstract = "Dentin sialoprotein (DSP) is essential for dentinogenesis and processed into fragments in the odontoblast-like cells and the tooth compartments. Matrix metalloproteinase 9 (MMP9) is expressed in teeth from early embryonic to adult stage. Although MMP9 has been reported to be involved in some physiological and pathological conditions through processing substrates, its role in tooth development and whether DSP is a substrate of MMP9 remain unknown. In this study, the function of MMP9 in the tooth development was examined by observation of Mmp9 knockout (Mmp9-/-) mouse phenotype, and whether DSP is a substrate of MMP9 was explored by in vitro and in vivo experiments. The results showed that Mmp9-/- teeth displayed a phenotype similar to dentinogenesis imperfecta, including decreased dentin mineral density, abnormal dentin architecture, widened predentin and irregular predentin-dentin boundary. The distribution of MMP9 and DSP overlapped in the odontoblasts, the predentin, and the mineralized dentin, and MMP9 was able to specifically bind to DSP. MMP9 highly efficiently cleaved DSP into distinct fragments in vitro, and the deletion of Mmp9 caused improper processing of DSP in natural teeth. Therefore, our findings demonstrate that MMP9 is important for tooth development and DSP is a novel target of MMP9 during dentinogenesis.",
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