Density gradient isolation of peripheral blood mononuclear cells using a blood cell processor

P. Law

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Large numbers of mononuclear cells (MNC) are needed for hematologic reconstitution using peripheral blood stem cells. The possibility of isolating those cells by discontinuous Ficoll‐diatrizoate density gradient centrifugation in two blood cell processors (the Haemonetics V50 [V50] and the Cobe 2991 [2991]) were examined. Buffy coats from peripheral blood containing 6.23 × 10(8) MNC were separated in the V50, resulting in a recovery of 75 percent. The purity of the cells, defined as the percentage of lymphocytes and monocytes among all leukocytes, was 95 percent. With larger cell loads (3 to 7 × 10(9) MNC), the yield was higher in the V50 than in the Cobe 2991 (92 versus 75%). After separation in the V50 or the 2991, the cloning efficiencies of hematopoietic progenitor cells (CFU‐GM and BFUe) were not different from those of cells isolated on 5 ml Ficoll‐diatrizoate gradients in centrifuge tubes. Both leukapheresis and MNC separation can be carried out with the same bowl and tubing set in the V50. With that approach, an average of 6 × 10(9) MNC were processed in 16 experiments. An average recovery of 82 percent with 95 percent purity was achieved. The authors conclude that, in terms of simplicity of operation, cost effectiveness, and maintenance of sterility, the V50 may be better suited than the 2991 for the purification of MNC from peripheral blood. 1988 AABB

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalTransfusion
Volume28
Issue number2
DOIs
StatePublished - Jan 1 1988

Fingerprint

Blood Cells
Blood Buffy Coat
Leukapheresis
Granulocyte-Macrophage Progenitor Cells
Density Gradient Centrifugation
Cell Separation
Hematopoietic Stem Cells
Infertility
Cost-Benefit Analysis
Organism Cloning
Monocytes
Leukocytes
Cell Count
Maintenance
Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Density gradient isolation of peripheral blood mononuclear cells using a blood cell processor. / Law, P.

In: Transfusion, Vol. 28, No. 2, 01.01.1988, p. 145-150.

Research output: Contribution to journalArticle

@article{5ed8de0ac2aa4ffab8e8c29f54440a81,
title = "Density gradient isolation of peripheral blood mononuclear cells using a blood cell processor",
abstract = "Large numbers of mononuclear cells (MNC) are needed for hematologic reconstitution using peripheral blood stem cells. The possibility of isolating those cells by discontinuous Ficoll‐diatrizoate density gradient centrifugation in two blood cell processors (the Haemonetics V50 [V50] and the Cobe 2991 [2991]) were examined. Buffy coats from peripheral blood containing 6.23 × 10(8) MNC were separated in the V50, resulting in a recovery of 75 percent. The purity of the cells, defined as the percentage of lymphocytes and monocytes among all leukocytes, was 95 percent. With larger cell loads (3 to 7 × 10(9) MNC), the yield was higher in the V50 than in the Cobe 2991 (92 versus 75{\%}). After separation in the V50 or the 2991, the cloning efficiencies of hematopoietic progenitor cells (CFU‐GM and BFUe) were not different from those of cells isolated on 5 ml Ficoll‐diatrizoate gradients in centrifuge tubes. Both leukapheresis and MNC separation can be carried out with the same bowl and tubing set in the V50. With that approach, an average of 6 × 10(9) MNC were processed in 16 experiments. An average recovery of 82 percent with 95 percent purity was achieved. The authors conclude that, in terms of simplicity of operation, cost effectiveness, and maintenance of sterility, the V50 may be better suited than the 2991 for the purification of MNC from peripheral blood. 1988 AABB",
author = "P. Law",
year = "1988",
month = "1",
day = "1",
doi = "10.1046/j.1537-2995.1988.28288179019.x",
language = "English (US)",
volume = "28",
pages = "145--150",
journal = "Transfusion",
issn = "0041-1132",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Density gradient isolation of peripheral blood mononuclear cells using a blood cell processor

AU - Law, P.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Large numbers of mononuclear cells (MNC) are needed for hematologic reconstitution using peripheral blood stem cells. The possibility of isolating those cells by discontinuous Ficoll‐diatrizoate density gradient centrifugation in two blood cell processors (the Haemonetics V50 [V50] and the Cobe 2991 [2991]) were examined. Buffy coats from peripheral blood containing 6.23 × 10(8) MNC were separated in the V50, resulting in a recovery of 75 percent. The purity of the cells, defined as the percentage of lymphocytes and monocytes among all leukocytes, was 95 percent. With larger cell loads (3 to 7 × 10(9) MNC), the yield was higher in the V50 than in the Cobe 2991 (92 versus 75%). After separation in the V50 or the 2991, the cloning efficiencies of hematopoietic progenitor cells (CFU‐GM and BFUe) were not different from those of cells isolated on 5 ml Ficoll‐diatrizoate gradients in centrifuge tubes. Both leukapheresis and MNC separation can be carried out with the same bowl and tubing set in the V50. With that approach, an average of 6 × 10(9) MNC were processed in 16 experiments. An average recovery of 82 percent with 95 percent purity was achieved. The authors conclude that, in terms of simplicity of operation, cost effectiveness, and maintenance of sterility, the V50 may be better suited than the 2991 for the purification of MNC from peripheral blood. 1988 AABB

AB - Large numbers of mononuclear cells (MNC) are needed for hematologic reconstitution using peripheral blood stem cells. The possibility of isolating those cells by discontinuous Ficoll‐diatrizoate density gradient centrifugation in two blood cell processors (the Haemonetics V50 [V50] and the Cobe 2991 [2991]) were examined. Buffy coats from peripheral blood containing 6.23 × 10(8) MNC were separated in the V50, resulting in a recovery of 75 percent. The purity of the cells, defined as the percentage of lymphocytes and monocytes among all leukocytes, was 95 percent. With larger cell loads (3 to 7 × 10(9) MNC), the yield was higher in the V50 than in the Cobe 2991 (92 versus 75%). After separation in the V50 or the 2991, the cloning efficiencies of hematopoietic progenitor cells (CFU‐GM and BFUe) were not different from those of cells isolated on 5 ml Ficoll‐diatrizoate gradients in centrifuge tubes. Both leukapheresis and MNC separation can be carried out with the same bowl and tubing set in the V50. With that approach, an average of 6 × 10(9) MNC were processed in 16 experiments. An average recovery of 82 percent with 95 percent purity was achieved. The authors conclude that, in terms of simplicity of operation, cost effectiveness, and maintenance of sterility, the V50 may be better suited than the 2991 for the purification of MNC from peripheral blood. 1988 AABB

UR - http://www.scopus.com/inward/record.url?scp=0023883843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023883843&partnerID=8YFLogxK

U2 - 10.1046/j.1537-2995.1988.28288179019.x

DO - 10.1046/j.1537-2995.1988.28288179019.x

M3 - Article

C2 - 3354042

AN - SCOPUS:0023883843

VL - 28

SP - 145

EP - 150

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 2

ER -