Denitration of 2,4,6-trinitrotoluene by Pseudomonas savastanoi

J. L. Martin, S. D. Comfort, P. J. Shea, T. A. Kokjohn, R. A. Drijber

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Past disposal of wastewaters containing 2,4,6-trinitrotoluene (TNT) at the former Nebraska Ordnance Plant has resulted in numerous acres of TNT- contaminated soil. Examining the microbial population of these soils revealed several TNT-tolerant Pseudomonas spp. We selected one species, P. savastanoi, to determine its ability to transform TNT. Pure culture experiments were performed in pseudomonas minimal medium containing 0.31 mM TNT (70 mg TNT · L-1) under varied nutrient and cell density regimes. Experiments with TNT as a sole C or N source showed that P. savastanoi has the ability to denitrate TNT, as evidenced by production of 2,4-dinitrotoluene (2,4-DNT) and NO2 with time. TNT denitration and formation of 2.4-DNT were enhanced by removing NH4/+ and adding NO2/- to the growth medium. In all experiments, 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT) appeared as incidental reduction products. Glucose addition to the medium enhanced 2-ADNT and 4-ADNT production and decreased denitration of TNT. Mid- log phase cells rapidly transformed [ring-14C(U)]TNT but were unable to mineralize significant quantities of TNT, as evidenced by conversion of less than 1% of the label to 14CO2. These results indicate that P. savastanoi is a TNT-tolerant pseudomonad that can promote TNT degradation through reductive denitration and nitro moiety reduction.

Original languageEnglish (US)
Pages (from-to)447-455
Number of pages9
JournalCanadian journal of microbiology
Volume43
Issue number5
DOIs
StatePublished - Jan 1 1997

Fingerprint

Trinitrotoluene
Denitrification
Pseudomonas
2,4-dinitrotoluene
2,6-dinitrotoluene
Soil
Soils
Ordnance
Experiments
Waste Water

Keywords

  • Biodegradation
  • Nitrite
  • Reduction
  • TNT
  • Transformation

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Denitration of 2,4,6-trinitrotoluene by Pseudomonas savastanoi. / Martin, J. L.; Comfort, S. D.; Shea, P. J.; Kokjohn, T. A.; Drijber, R. A.

In: Canadian journal of microbiology, Vol. 43, No. 5, 01.01.1997, p. 447-455.

Research output: Contribution to journalArticle

Martin, J. L. ; Comfort, S. D. ; Shea, P. J. ; Kokjohn, T. A. ; Drijber, R. A. / Denitration of 2,4,6-trinitrotoluene by Pseudomonas savastanoi. In: Canadian journal of microbiology. 1997 ; Vol. 43, No. 5. pp. 447-455.
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