3 Citations (Scopus)

Abstract

Dendritic cells (DCs) are critical to the initiation of a T-cell response. They constitute the most potent antigen-presenting cell (APC) endowed with the unique capacity to stimulate an antigen-specific T-cell responses by naive T cells. Adenoviruses (Ad) have high transduction efficiency for many cell types including cells of hematopoietic origin independent of their mitotic status, and replication-defective Ad have demonstrated a safety profile clinically. Further, Ad vectors provide a high level of transgene expression, and Ad-transduced DCs can effectively present antigenic proteins. In this chapter, we outline a functionally closed, good manufacturing protocol for the differentiation of monocytes into DCs and transduction by Ad vectors. Basic functional and phenotypic release assays are provided, as well as contrasting research approaches for Ad-transduced DC-based vaccines.

Original languageEnglish (US)
Pages (from-to)97-118
Number of pages22
JournalMethods in Molecular Biology
Volume1139
DOIs
StatePublished - Jan 1 2014

Fingerprint

Adenoviridae
Dendritic Cells
Vaccines
T-Lymphocytes
Antigen-Presenting Cells
Transgenes
Monocytes
Safety
Antigens
Research
Proteins

Keywords

  • Adenovirus
  • Dendritic cells
  • Monocytes
  • Release assays
  • Vaccine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Dendritic cells transfected with adenoviral vectors as vaccines. / Senesac, Joseph; Gabrilovich, Dmitry; Pirruccello, Samuel Jay; Talmadge, James E.

In: Methods in Molecular Biology, Vol. 1139, 01.01.2014, p. 97-118.

Research output: Contribution to journalArticle

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T1 - Dendritic cells transfected with adenoviral vectors as vaccines

AU - Senesac, Joseph

AU - Gabrilovich, Dmitry

AU - Pirruccello, Samuel Jay

AU - Talmadge, James E

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