Delivery of the interleukin-1 type ii receptor cdna inhibits interleurin-8 production in human fibroblasts

D. L. Knoell, C. B. Marsh, M. D. Wewers

Research output: Contribution to journalArticle

Abstract

Communication between monocytes, fibroblasts, and hepatocytes in the liver constitute an important feedback pathway in inflammation. Interleukin-1 (ILI) is a key mediator in all three cell types and is directly involved in the liver response to inflammatory stimuli. Recent evidence suggests that the Interleukin-1 Type II receptor (IL-1 Ilr) serves as an endogenous inhibitor of IL-1. We hypothesized that overexpression of the soluble IL-1 Ilr in hepatocytes would inhibit IL-1β mediated inflammation by hepatocytes, fibroblasts, and monocytes. To test this hypothesis, transfection of the human hepatoma cell line HepG2 was optimized using a β-galactosidase reporter plasmid and Lipofectamine®, A mammalian expression vector containing the human IL-1 Ilr cDNA (provided by U.Gubler) was then used to transfect HepG2 cells. Soluble IL-1 Ilr was detectable by ELISA in the supernatents of transfected HepG2 cells (1500-4500 pg/ml) 3-5 days after transfection. The same supernatents were found to bind IL-1 as determined by decreased IL-I ïïr ELISA detection in the presence of recombinant IL-1β. When transfected HepG2 supernatents were incubated with human gingival fibroblasts in the presence of IL-1β (100 pg/ml), interleukin-8 production was completely inhibited (as measured by ELISA in supernatents) whereas, IL-8 induction was unaffected in fibroblasts treated with control and sham-transfected conditioned médias (ANOVA p=.001). IL-1 induction of IL-8 in HepG2 cells and human monocytes was not inhibited by the presence of soluble IL-1 Ilr from transfected cells. These results suggest that the inhibitory effects of soluble IL-1 Ilr are cell dependent and demonstrate the potential for gene delivery to parenchymal cells of the liver to inhibit cytokine mediated inflammation.

Original languageEnglish (US)
Pages (from-to)A1331
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

Fingerprint

Interleukin-1 Receptors
interleukin-1
Fibroblasts
Interleukin-1
fibroblasts
receptors
hepatocytes
Hep G2 Cells
interleukin-8
Interleukin-8
Liver
monocytes
Monocytes
Hepatocytes
Enzyme-Linked Immunosorbent Assay
inflammation
Inflammation
enzyme-linked immunosorbent assay
cells
transfection

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Delivery of the interleukin-1 type ii receptor cdna inhibits interleurin-8 production in human fibroblasts. / Knoell, D. L.; Marsh, C. B.; Wewers, M. D.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996, p. A1331.

Research output: Contribution to journalArticle

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abstract = "Communication between monocytes, fibroblasts, and hepatocytes in the liver constitute an important feedback pathway in inflammation. Interleukin-1 (ILI) is a key mediator in all three cell types and is directly involved in the liver response to inflammatory stimuli. Recent evidence suggests that the Interleukin-1 Type II receptor (IL-1 Ilr) serves as an endogenous inhibitor of IL-1. We hypothesized that overexpression of the soluble IL-1 Ilr in hepatocytes would inhibit IL-1β mediated inflammation by hepatocytes, fibroblasts, and monocytes. To test this hypothesis, transfection of the human hepatoma cell line HepG2 was optimized using a β-galactosidase reporter plasmid and Lipofectamine{\circledR}, A mammalian expression vector containing the human IL-1 Ilr cDNA (provided by U.Gubler) was then used to transfect HepG2 cells. Soluble IL-1 Ilr was detectable by ELISA in the supernatents of transfected HepG2 cells (1500-4500 pg/ml) 3-5 days after transfection. The same supernatents were found to bind IL-1 as determined by decreased IL-I {\"i}{\"i}r ELISA detection in the presence of recombinant IL-1β. When transfected HepG2 supernatents were incubated with human gingival fibroblasts in the presence of IL-1β (100 pg/ml), interleukin-8 production was completely inhibited (as measured by ELISA in supernatents) whereas, IL-8 induction was unaffected in fibroblasts treated with control and sham-transfected conditioned m{\'e}dias (ANOVA p=.001). IL-1 induction of IL-8 in HepG2 cells and human monocytes was not inhibited by the presence of soluble IL-1 Ilr from transfected cells. These results suggest that the inhibitory effects of soluble IL-1 Ilr are cell dependent and demonstrate the potential for gene delivery to parenchymal cells of the liver to inhibit cytokine mediated inflammation.",
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