Deletions in the second stalk of F1F0-ATP synthase in Escherichia coli

Paul L Sorgen, Tamara L. Caviston, Regina C. Perry, Brian D. Cain

Research output: Contribution to journalArticle

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Abstract

In Escherichia coli F1F0-ATP synthase, the two b subunits form the second stalk spanning the distance between the membrane F0 sector and the bulk of F1. Current models predict that the stator should be relatively rigid and engaged in contact with F1 at fixed points. To test this hypothesis, we constructed a series of deletion mutations in the uncF(b) gene to remove segments from the middle of the second stalk of the subunit. Mutants with deletions of 7 amino acids were essentially normal, and those with deletions of up to 11 amino acids retained considerable activity. Membranes prepared from these strains had readily detectable levels of F1- ATPase activity and proton pumping activity. Removal of 12 or more amino acids resulted in loss of oxidative phosphorylation. Levels of membrane- associated F1-ATPase dropped precipitously for the longer deletions, and immunoblot analysis indicated that reductions in activity correlated with reduced levels of b subunit in the membranes. Assuming the likely α-helical conformation for this area of the b subunit, the 11-amino acid deletion would result in shortening the subunit by approximately 16 Å. Since these deletions did not prevent the b subunit from participating in productive interactions with F1, we suggest that the b subunit is not a rigid rodlike structure, but has an inherent flexibility compatible with a dynamic role in coupling.

Original languageEnglish (US)
Pages (from-to)27873-27878
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number43
DOIs
StatePublished - Oct 23 1998

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Escherichia coli
Membranes
Amino Acids
Proton-Translocating ATPases
Rigid structures
Sequence Deletion
Oxidative Phosphorylation
Stators
Conformations
Protons
Genes
F1F0-ATP synthase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Deletions in the second stalk of F1F0-ATP synthase in Escherichia coli. / Sorgen, Paul L; Caviston, Tamara L.; Perry, Regina C.; Cain, Brian D.

In: Journal of Biological Chemistry, Vol. 273, No. 43, 23.10.1998, p. 27873-27878.

Research output: Contribution to journalArticle

Sorgen, Paul L ; Caviston, Tamara L. ; Perry, Regina C. ; Cain, Brian D. / Deletions in the second stalk of F1F0-ATP synthase in Escherichia coli. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 43. pp. 27873-27878.
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