Degradation of electrospun PLGA-chitosan/PVA membranes and their cytocompatibility in vitro

Bin Duan, Lili Wu, Xiaoran Li, Xiaoyan Yuan, Xiulan Li, Yang Zhang, Kangde Yao

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Nanofibrious composite poly(lactide-co-glycolide) (PLGA) and chitosan/ poly(vinyl alcohol) (PVA) membranes were prepared by simultaneously electrospinning PLGA and chitosan/PVA from two different syringes. The in vitro degradation of PLGA and cross-linked composite membranes was examined for up to 10 weeks in phosphate-buffered saline (PBS, pH 7.4) at 37°C. The pH of PBS, the weight average molecular weight of PLGA, fiber morphology and mechanical properties, including tensile strength, Young's modulus and elongation-at-break, were measured as a function of degradation time. The fibrous composite membranes were further investigated as a promising scaffold for human embryo skin fibroblasts (hESFs) culture. The cell adhesion and morphology of hESFs seeded on each electrospun membrane was observed using scanning electron microscope and inverted phase contrast microscopy after Wright-Giemsa staining. The introduction of chitosan/PVA component changed the hydrophilic/hydrophobic balance and, thus, influenced degradation behavior and mechanical properties of the composite membranes during degradation. The cells could not only favorably attach and grow well on the composite membranes, but were also able to migrate and infiltrate the membranes. Therefore, the results suggest that the composite membranes can positively mimic the structure of natural extracellular matrices and have the potential for application as three-dimensional tissue-engineering scaffolds.

Original languageEnglish (US)
Pages (from-to)95-115
Number of pages21
JournalJournal of Biomaterials Science, Polymer Edition
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Composite membranes
Chitosan
Membranes
Degradation
Fibroblasts
Scaffolds (biology)
Skin
Fibrous membranes
Tissue Scaffolds
Polyglactin 910
Syringes
Mechanical properties
Cell adhesion
Bioelectric potentials
Electrospinning
Tissue engineering
Cell culture
Embryonic Structures
Elongation
Microscopic examination

Keywords

  • Chitosan
  • Cytocompatibility
  • Electrospinning
  • In vitro degradation
  • Poly(lactide-co-glycolide)

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Degradation of electrospun PLGA-chitosan/PVA membranes and their cytocompatibility in vitro. / Duan, Bin; Wu, Lili; Li, Xiaoran; Yuan, Xiaoyan; Li, Xiulan; Zhang, Yang; Yao, Kangde.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 18, No. 1, 01.01.2007, p. 95-115.

Research output: Contribution to journalArticle

Duan, Bin ; Wu, Lili ; Li, Xiaoran ; Yuan, Xiaoyan ; Li, Xiulan ; Zhang, Yang ; Yao, Kangde. / Degradation of electrospun PLGA-chitosan/PVA membranes and their cytocompatibility in vitro. In: Journal of Biomaterials Science, Polymer Edition. 2007 ; Vol. 18, No. 1. pp. 95-115.
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