Deficiency of Prdx6 in lens epithelial cells induces ER stress response-mediated impaired homeostasis and apoptosis

Nigar Fatma, Prerna Singh, Bhavana Chhunchha, Eri Kubo, T. Shinohara, Biju Bhargavan, Dhirendra P. Singh

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The multifunctional cytoprotective protein peroxiredoxin 6 (Prdx6) maintains cellular homeostasis and membrane integrity by regulating expression of intracellular reactive oxygen species (ROS) and phospholipid turnover. Using cells derived from targeted inactivation of Prdx6 gene or its depletion by RNA interference or aging, we showed that Prdx6 deficiency in cells evoked unfolded protein response (UPR), evidenced by increased expression or activation of proapoptotic factors, CHOP, ATF4, PERK, IRE-α and eIF2-α and by increased caspases 3 and 12 processing. Those cells displayed enhanced and sustained expression of endoplasmic reticulum (ER) stress-related chaperon proteins, Bip/glucose-regulated protein 78, calnexin, and calreticulin. Under cellular stress induced by hypoxia (1% O 2 or CoC l2 treatment) or tunicamycin, Prdx6-deficient cells exhibited aberrant activation of ER stress-responsive genes/protein with higher expression of ROS, and died with apoptosis. Wild-type cells exposed to tunicamycin or hypoxia remained relatively insensitive with lower expression of ROS and ER-responsive genes than did Prdx6-deficient cells, but upregulation of ER stress responsive proteins or chaperones mimicked the UPR response of Prdx6-deficient or aging cells. Expression of Prdx6 blocked ER stress-induced deleterious signaling by optimizing physiologically aberrant expression of ER stress responsive genes/proteins in Prdx6-deficient cells or cells facing stressors, and rescued the cells from apoptosis. These findings demonstrate that impaired homeostasis and progression of pathogenesis in Prdx6-deficient lens epithelial cells or in aging cells should be blocked by a supply of Prdx6. The results provide a new molecular basis for understanding the etiology of several age-associated degenerative disorders, and potentially for developing antioxidant Prdx6-based therapeutics.

Original languageEnglish (US)
Pages (from-to)C954-C967
JournalAmerican Journal of Physiology - Cell Physiology
Volume301
Issue number4
DOIs
StatePublished - Oct 1 2011

Fingerprint

Peroxiredoxin VI
Endoplasmic Reticulum Stress
Lenses
Homeostasis
Epithelial Cells
Apoptosis
Unfolded Protein Response
Tunicamycin
Reactive Oxygen Species
Cell Aging
Proteins
Caspase 12
Calnexin
Calreticulin
Heat-Shock Proteins
RNA Interference
Caspase 3
Endoplasmic Reticulum

Keywords

  • Antioxidant
  • Endoplasmic reticulum
  • Peroxiredoxin 6
  • Reactive oxygen species
  • Unfolded protein response

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Deficiency of Prdx6 in lens epithelial cells induces ER stress response-mediated impaired homeostasis and apoptosis. / Fatma, Nigar; Singh, Prerna; Chhunchha, Bhavana; Kubo, Eri; Shinohara, T.; Bhargavan, Biju; Singh, Dhirendra P.

In: American Journal of Physiology - Cell Physiology, Vol. 301, No. 4, 01.10.2011, p. C954-C967.

Research output: Contribution to journalArticle

Fatma, Nigar ; Singh, Prerna ; Chhunchha, Bhavana ; Kubo, Eri ; Shinohara, T. ; Bhargavan, Biju ; Singh, Dhirendra P. / Deficiency of Prdx6 in lens epithelial cells induces ER stress response-mediated impaired homeostasis and apoptosis. In: American Journal of Physiology - Cell Physiology. 2011 ; Vol. 301, No. 4. pp. C954-C967.
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AU - Bhargavan, Biju

AU - Singh, Dhirendra P.

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