Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease

Kim A. Brogden, M. Heidari, R. E. Sacco, D. Palmquist, Janet M Guthmiller, G. K. Johnson, H. P. Jia, B. F. Tack, P. B. McCray

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The severity of periodontal disease is dependent on a combination of host, microbial agent and environmental factors. One strong correlate related to periodontal disease pathogenesis is the immune status of the host. Here we show that human neutrophil peptide (HNP) defensins or human β-defensins (HBD), co-administered intranasally with the antigen ovalbumin (OVA), induce unique immune responses that if used with microbial antigens may have the potential to hinder the pathogenesis of periodontal disease. C57BL/6 mice were immunized intranasally with phosphate buffered saline (PBS) containing 1 μg HNP-1, HNP-2, HBD1 or HBD2 with and without 50 μg OVA. At 21 days, isotypes and subclasses of OVA-specific antibodies were determined in saliva, serum, nasal wash, bronchoalveolar lavage fluid, and fecal extracts. OVA-stimulated splenic lymphoid cell cultures from immunized mice were assessed for interferon (IFN)-γ, Interleukin (IL)-4 and IL-10. In comparison with mice immunized with only OVA, HNP-1 and HBD2 induced significantly higher (P < 0.05) OVA-specific serum IgG, lower, but not significant, serum IgM and significantly lower (P < 0.05) IFN-γ. In contrast, HNP-2 induced low OVA-specific serum IgG and higher, but not significant, serum IgM. HBD1 induced significantly higher (P < 0.05) OVA-specific serum IgG, higher, but not significant, serum IgM, and significantly higher (P < 0.05) IL-10. The elevated serum IgG subclasses contained IgG1 and IgG2b.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalOral Microbiology and Immunology
Volume18
Issue number2
DOIs
StatePublished - Apr 1 2003

Fingerprint

Defensins
Ovalbumin
Periodontal Diseases
Adaptive Immunity
Serum
Immunoglobulin G
Immunoglobulin M
Interleukin-10
Interferons
Antigens
Bronchoalveolar Lavage Fluid
Inbred C57BL Mouse
Nose
Saliva
Interleukin-4
Neutrophils
Cell Culture Techniques
Phosphates
Lymphocytes
Peptides

Keywords

  • Adaptive immunity
  • Defensins
  • Host response
  • Innate immunity

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

Cite this

Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease. / Brogden, Kim A.; Heidari, M.; Sacco, R. E.; Palmquist, D.; Guthmiller, Janet M; Johnson, G. K.; Jia, H. P.; Tack, B. F.; McCray, P. B.

In: Oral Microbiology and Immunology, Vol. 18, No. 2, 01.04.2003, p. 95-99.

Research output: Contribution to journalArticle

Brogden, KA, Heidari, M, Sacco, RE, Palmquist, D, Guthmiller, JM, Johnson, GK, Jia, HP, Tack, BF & McCray, PB 2003, 'Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease', Oral Microbiology and Immunology, vol. 18, no. 2, pp. 95-99. https://doi.org/10.1034/j.1399-302X.2003.00047.x
Brogden, Kim A. ; Heidari, M. ; Sacco, R. E. ; Palmquist, D. ; Guthmiller, Janet M ; Johnson, G. K. ; Jia, H. P. ; Tack, B. F. ; McCray, P. B. / Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease. In: Oral Microbiology and Immunology. 2003 ; Vol. 18, No. 2. pp. 95-99.
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