Defective transport of hemagglutinin-neuraminidase glycoprotein of bovine parainfluenza-3 virus in interferon treated cell

Pinaki Panigrahi, Sashi B. Mohanty

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A defective transport of bovine parainfluenza-3 virus (PI-3V) hemagglutinin-neuraminidase (HN) glycoprotein was evidenced in interferon (IFN)-treated bovine turbinate (BTu) cells. Indirect immunofluorescence performed with monoclonal antibody to PI-3 HN glycoprotein demonstrated accumulation of this protein in the perinuclear cytoplasm of IFN-treated cells. Untreated, infected control cells had a generalized widespread fluorescence. Unfixed control cells showed a uniform surface fluorescence in contrast to a few specs of fluorescence on the plasma membrane of IFN-treated cells. Electron microscopic localization of HN protein was done by immuno-gold ultrastructural cytochemistry. Untreated cells had uniform gold label on the plasma membrane and around the budding virus particles with no label in the cytoplasm. In IFN-treated cells, however, there was an accumulation of gold particles in the cytoplasm with only a few particles on the cell surface. Quantitative analysis of HN protein on the cell surface by solid phase radioimmune-assay revealed a greater amount of this protein on the surface of control cells, than those on the IFN-treated cells.

Original languageEnglish (US)
Pages (from-to)125-133
Number of pages9
JournalArchives of Virology
Volume109
Issue number1-2
DOIs
StatePublished - Mar 1 1989

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Bovine parainfluenza virus 3
HN Protein
Interferons
Gold
Cytoplasm
Fluorescence
Hemagglutinins
Neuraminidase
Membrane Proteins
Cell Membrane
Turbinates
Histocytochemistry
Indirect Fluorescent Antibody Technique
Virion

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Defective transport of hemagglutinin-neuraminidase glycoprotein of bovine parainfluenza-3 virus in interferon treated cell. / Panigrahi, Pinaki; Mohanty, Sashi B.

In: Archives of Virology, Vol. 109, No. 1-2, 01.03.1989, p. 125-133.

Research output: Contribution to journalArticle

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