Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production

Li Hua Zhu, Frans Krens, Mark A. Smith, Xueyuan Li, Weicong Qi, Eibertus N. Van Loo, Tim Iven, Ivo Feussner, Tara J. Nazarenus, Dongxin Huai, David C. Taylor, Xue Rong Zhou, Allan G. Green, Jay Shockey, K. Thomas Klasson, Robert T. Mullen, Bangquan Huang, John M. Dyer, Edgar B. Cahoon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.

Original languageEnglish (US)
Article number22181
JournalScientific reports
Volume6
DOIs
StatePublished - Feb 26 2016

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Industrial Oils
Metabolic Engineering
Waxes
Lubricants
Esters
Plant Oils
Fatty Acids
Brassicaceae
Petroleum
Biotechnology
Seeds
Polymers
Oils
Down-Regulation
Food
Temperature

ASJC Scopus subject areas

  • General

Cite this

Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production. / Zhu, Li Hua; Krens, Frans; Smith, Mark A.; Li, Xueyuan; Qi, Weicong; Van Loo, Eibertus N.; Iven, Tim; Feussner, Ivo; Nazarenus, Tara J.; Huai, Dongxin; Taylor, David C.; Zhou, Xue Rong; Green, Allan G.; Shockey, Jay; Klasson, K. Thomas; Mullen, Robert T.; Huang, Bangquan; Dyer, John M.; Cahoon, Edgar B.

In: Scientific reports, Vol. 6, 22181, 26.02.2016.

Research output: Contribution to journalArticle

Zhu, LH, Krens, F, Smith, MA, Li, X, Qi, W, Van Loo, EN, Iven, T, Feussner, I, Nazarenus, TJ, Huai, D, Taylor, DC, Zhou, XR, Green, AG, Shockey, J, Klasson, KT, Mullen, RT, Huang, B, Dyer, JM & Cahoon, EB 2016, 'Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production', Scientific reports, vol. 6, 22181. https://doi.org/10.1038/srep22181
Zhu, Li Hua ; Krens, Frans ; Smith, Mark A. ; Li, Xueyuan ; Qi, Weicong ; Van Loo, Eibertus N. ; Iven, Tim ; Feussner, Ivo ; Nazarenus, Tara J. ; Huai, Dongxin ; Taylor, David C. ; Zhou, Xue Rong ; Green, Allan G. ; Shockey, Jay ; Klasson, K. Thomas ; Mullen, Robert T. ; Huang, Bangquan ; Dyer, John M. ; Cahoon, Edgar B. / Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production. In: Scientific reports. 2016 ; Vol. 6.
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