Decreased stability of transforming growth factor β type II receptor mRNA in RER+ human colon carcinoma cells

Wen Jiang, Manoranjani P.M. Tillekeratne, Michael G. Brattain, Sunandita S. Banerji

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Abstract

Transforming growth factor β (TGF-β) is a potent inhibitor of cell growth and tumor progression. Previous work has shown that loss of functional TGF-β type II receptor (RII) due to a frameshift mutation in the 5' half of the RII gene leads to TGF-β resistance in a highly progressed, RER+ human colon carcinoma cell line designated HCT116. Expression of this mutated RII gene was highly repressed in RER+ cell lines such as HCTI 16 and RKO, as analyzed by RNase protection assays. Nuclear run-on and RII promoter-reporter (CAT) assays showed that the transcriptional levels of the RII gene in these RER+ cells were not reduced, compared to RII-expressing cells. However, the half-lives of the RII mRNA, as analyzed by RNase protection assays following actinomycin D treatment, were significantly decreased. This suggested that the decreased expression of the RII gene mutant was due to decreased mRNA stability. Furthermore, RII mRNA from HCT116 transfected with wild-type RII had a longer half-life than the endogenous mutated RII mRNA. A dominant negative RII mutant, which encodes a similarly truncated RII protein as HCT116 but lacks the extensive 3' untranslated region of RII mRNA, gave the same half-life as endogenous wild-type RII mRNA. We conclude that the frameshift mutation which results in a premature stop codon in the 5' half of the mRNA transcript accounts for the reduced RII mRNA levels in RER+ cells.

Original languageEnglish (US)
Pages (from-to)14786-14793
Number of pages8
JournalBiochemistry
Volume36
Issue number48
DOIs
Publication statusPublished - Dec 2 1997

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ASJC Scopus subject areas

  • Biochemistry

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