Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins

Andrew A. Potter, Sandra Klashinsky, Yuling Li, Elizabeth Frey, Hugh Townsend, Dragan Rogan, Galen Erickson, Susanne Hinkley, Terry Klopfenstein, Rodney A Moxley, David R. Smith, B. Brett Finlay

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

Cattle are an important reservoir of Escherichia coli O157:H7 leading to contamination of food and water, and subsequent human disease. This pathogen colonizes its hosts by producing several proteins such as Tir and EspA that are secreted by a type III secretion system. These proteins play a role in colonization of the intestine, suggesting that they might be useful targets for the development of a vaccine to reduce levels of this organism in cattle. Vaccination of cattle with proteins secreted by E. coli O157:H7 significantly reduced the numbers of bacteria shed in feces, the numbers of animals that shed, and the duration of shedding in an experimental challenge model. Vaccination of cattle also significantly (P=0.04) reduced the prevalence of E. coli O157:H7 in a clinical trial conducted in a typical feedlot setting. This strategy suggests it is possible to vaccinate cattle to decrease the level of E. coli O157:H7 shedding for the purpose of reducing the risk of human disease.

Original languageEnglish (US)
Pages (from-to)362-369
Number of pages8
JournalVaccine
Volume22
Issue number3-4
DOIs
StatePublished - Jan 2 2004

Fingerprint

Escherichia coli O157
Vaccination
vaccination
cattle
Proteins
human diseases
proteins
Food Contamination
type III secretion system
vaccine development
food contamination
water pollution
feedlots
Feces
Intestines
clinical trials
intestines
Theoretical Models
Vaccines
feces

Keywords

  • Bovine vaccine
  • EHEC
  • O157:H7

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Potter, A. A., Klashinsky, S., Li, Y., Frey, E., Townsend, H., Rogan, D., ... Finlay, B. B. (2004). Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins. Vaccine, 22(3-4), 362-369. https://doi.org/10.1016/j.vaccine.2003.08.007

Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins. / Potter, Andrew A.; Klashinsky, Sandra; Li, Yuling; Frey, Elizabeth; Townsend, Hugh; Rogan, Dragan; Erickson, Galen; Hinkley, Susanne; Klopfenstein, Terry; Moxley, Rodney A; Smith, David R.; Finlay, B. Brett.

In: Vaccine, Vol. 22, No. 3-4, 02.01.2004, p. 362-369.

Research output: Contribution to journalArticle

Potter, AA, Klashinsky, S, Li, Y, Frey, E, Townsend, H, Rogan, D, Erickson, G, Hinkley, S, Klopfenstein, T, Moxley, RA, Smith, DR & Finlay, BB 2004, 'Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins', Vaccine, vol. 22, no. 3-4, pp. 362-369. https://doi.org/10.1016/j.vaccine.2003.08.007
Potter, Andrew A. ; Klashinsky, Sandra ; Li, Yuling ; Frey, Elizabeth ; Townsend, Hugh ; Rogan, Dragan ; Erickson, Galen ; Hinkley, Susanne ; Klopfenstein, Terry ; Moxley, Rodney A ; Smith, David R. ; Finlay, B. Brett. / Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins. In: Vaccine. 2004 ; Vol. 22, No. 3-4. pp. 362-369.
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