Decreased plakophilin-1 expression promotes increased motility in head and neck squamous cell carcinoma cells

Tammy Sobolik-Delmaire, Dawn Katafiasz, Sarah A. Keim, My G. Mahoney, James K Wahl

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Desmosomes are prominent cell-cell adhesive junctions found in a variety of epithelial tissues, including the oral epithelium. The transmembrane core of the desmosome is composed of the desmosomal cadherins that interact extracellularly to mediate cell-cell adhesion. The cytoplasmic domain of desmosomal cadherins interact with plaque proteins that in turn interact with the keratin intermediate filament cytoskeleton. Plakophilin 1 is a major desmosomal plaque component that functions to recruit intermediate filaments to sites of cell-cell contact via interactions with desmoplakin. Decreased assembly of desmosomes has been reported in several epithelial cancers. We examined plakophilin-1 expression in an esophageal squamous cell carcinoma tissue microarray and found that plakophilin-1 expression inversely correlates with tumor grade. In addition, we sought to investigate the effect of plakophilin-1 expression on desmosome assembly and cell motility in oral squamous cell carcinoma cell lines. Cell lines expressing altered levels of plakophilin-1 were generated and the ability of these cells to recruit desmoplakin to sites of cell-cell contact was examined. Our results show that decreased expression of plakophilin-1 results in decreased desmosome assembly and increased cell motility and invasion. These data lead us to propose that loss of plakophilin-1 expression during head and neck squamous cell carcinoma (HNSCC) progression may contribute to an invasive phenotype.

Original languageEnglish (US)
Pages (from-to)99-109
Number of pages11
JournalCell Communication and Adhesion
Volume14
Issue number2-3
DOIs
StatePublished - Mar 1 2007

Fingerprint

Plakophilins
Desmosomes
Cells
Desmosomal Cadherins
Desmoplakins
Intermediate Filaments
Cell Movement
Epithelium
Tissue
Cell Line
Intercellular Junctions
Cell adhesion
Microarrays
Keratins
Carcinoma, squamous cell of head and neck
Epithelial Cells
Cytoskeleton
Cell Adhesion
Adhesives
Tumors

Keywords

  • Cell-cell adhesion
  • Desmosomes
  • Motility
  • Plakophilin-1

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Decreased plakophilin-1 expression promotes increased motility in head and neck squamous cell carcinoma cells. / Sobolik-Delmaire, Tammy; Katafiasz, Dawn; Keim, Sarah A.; Mahoney, My G.; Wahl, James K.

In: Cell Communication and Adhesion, Vol. 14, No. 2-3, 01.03.2007, p. 99-109.

Research output: Contribution to journalArticle

Sobolik-Delmaire, Tammy ; Katafiasz, Dawn ; Keim, Sarah A. ; Mahoney, My G. ; Wahl, James K. / Decreased plakophilin-1 expression promotes increased motility in head and neck squamous cell carcinoma cells. In: Cell Communication and Adhesion. 2007 ; Vol. 14, No. 2-3. pp. 99-109.
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