Decreased expression of cellular prostatic acid phosphatase increases tumorigenicity of human prostate cancer cells

Ming-Fong Lin, Ming Shyue Lee, Xiao Wei Zhou, John C. Andressen, Tzu Ching Meng, Sonny L. Johansson, William W. West, Rodney J. Taylor, James R. Anderson, Fen Fen Lin

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Purpose: Understanding cell proliferation regulation in hormone refractory prostate cancer may provide answers for novel solutions. Protein tyrosine phosphatases have been thought to have key roles in regulating cell proliferation and be involved in oncogenesis, although to our knowledge their functional roles in human prostate cancer remain unknown. Human prostatic acid phosphatase (PAcP), a major phosphatase in prostate epithelium, has been shown to function as a neutral protein tyrosine phosphatase in these cells. We evaluated the biological significance of cellular prostatic acid phosphatase expression in human prostate cancer cells. Materials and Methods: Immunohistochemical testing of human prostate cancer archival specimens was done to evaluate the expression of cellular PAcP. Immunoprecipitation and immunoblotting were performed to determine cellular PAcP and SH2 domain-bearing tyrosine phosphatase-1 levels as well as tyrosine phosphorylation of c-ErbB-2/neu in different human prostate cancer cells. The biological behavior of LNCaP derivative sublines was characterized in vitro and in vivo by soft agar analysis and xenograft animal inoculation. Results: Immunohistochemical staining of human prostate clearly showed that cellular levels of PAcP significantly decreases in prostate cancer cells (p<0.001). The results of biochemical characterization revealed that the cellular level of PAcP but not SHP-1, another differentiation associated protein tyrosine phosphatase, consistently correlated negatively with the growth of several human prostate cancer cell lines. Reintroducing cellular PAcP activity in prostate cancer cells by PAcP complementary DNA transfection resulted in decreased tyrosine phosphorylation of c-ErbB-2/neu, decreased proliferation rates in culture as well as decreased anchorage independent growth in soft agar. The xenograft animal model demonstrated that a higher tumor growth rate as well as larger size is associated with a lower level of cellular PAcP. Conclusions: Cellular PAcP can down-regulate prostate cancer cell growth, at least partially by dephosphorylating c-ErbB-2/neu. Therefore, decreased cellular PAcP expression in cancer cells may be involved in prostate cancer progression.

Original languageEnglish (US)
Pages (from-to)1943-1950
Number of pages8
JournalJournal of Urology
Volume166
Issue number5
DOIs
StatePublished - Jan 1 2001

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Prostatic Neoplasms
Protein Tyrosine Phosphatases
Tyrosine
Growth
Phosphoric Monoester Hydrolases
Heterografts
Agar
Prostate
prostatic acid phosphatase
Phosphorylation
Cell Proliferation
src Homology Domains
Immunoprecipitation
Immunoblotting
Transfection
Neoplasms
Carcinogenesis
Down-Regulation
Epithelium
Animal Models

Keywords

  • Disease progression
  • Prostate
  • Prostatic neoplasms
  • Protein-tyrosine-phosphatase

ASJC Scopus subject areas

  • Urology

Cite this

Decreased expression of cellular prostatic acid phosphatase increases tumorigenicity of human prostate cancer cells. / Lin, Ming-Fong; Lee, Ming Shyue; Zhou, Xiao Wei; Andressen, John C.; Meng, Tzu Ching; Johansson, Sonny L.; West, William W.; Taylor, Rodney J.; Anderson, James R.; Lin, Fen Fen.

In: Journal of Urology, Vol. 166, No. 5, 01.01.2001, p. 1943-1950.

Research output: Contribution to journalArticle

Lin, M-F, Lee, MS, Zhou, XW, Andressen, JC, Meng, TC, Johansson, SL, West, WW, Taylor, RJ, Anderson, JR & Lin, FF 2001, 'Decreased expression of cellular prostatic acid phosphatase increases tumorigenicity of human prostate cancer cells', Journal of Urology, vol. 166, no. 5, pp. 1943-1950. https://doi.org/10.1016/S0022-5347(05)65725-4
Lin, Ming-Fong ; Lee, Ming Shyue ; Zhou, Xiao Wei ; Andressen, John C. ; Meng, Tzu Ching ; Johansson, Sonny L. ; West, William W. ; Taylor, Rodney J. ; Anderson, James R. ; Lin, Fen Fen. / Decreased expression of cellular prostatic acid phosphatase increases tumorigenicity of human prostate cancer cells. In: Journal of Urology. 2001 ; Vol. 166, No. 5. pp. 1943-1950.
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abstract = "Purpose: Understanding cell proliferation regulation in hormone refractory prostate cancer may provide answers for novel solutions. Protein tyrosine phosphatases have been thought to have key roles in regulating cell proliferation and be involved in oncogenesis, although to our knowledge their functional roles in human prostate cancer remain unknown. Human prostatic acid phosphatase (PAcP), a major phosphatase in prostate epithelium, has been shown to function as a neutral protein tyrosine phosphatase in these cells. We evaluated the biological significance of cellular prostatic acid phosphatase expression in human prostate cancer cells. Materials and Methods: Immunohistochemical testing of human prostate cancer archival specimens was done to evaluate the expression of cellular PAcP. Immunoprecipitation and immunoblotting were performed to determine cellular PAcP and SH2 domain-bearing tyrosine phosphatase-1 levels as well as tyrosine phosphorylation of c-ErbB-2/neu in different human prostate cancer cells. The biological behavior of LNCaP derivative sublines was characterized in vitro and in vivo by soft agar analysis and xenograft animal inoculation. Results: Immunohistochemical staining of human prostate clearly showed that cellular levels of PAcP significantly decreases in prostate cancer cells (p<0.001). The results of biochemical characterization revealed that the cellular level of PAcP but not SHP-1, another differentiation associated protein tyrosine phosphatase, consistently correlated negatively with the growth of several human prostate cancer cell lines. Reintroducing cellular PAcP activity in prostate cancer cells by PAcP complementary DNA transfection resulted in decreased tyrosine phosphorylation of c-ErbB-2/neu, decreased proliferation rates in culture as well as decreased anchorage independent growth in soft agar. The xenograft animal model demonstrated that a higher tumor growth rate as well as larger size is associated with a lower level of cellular PAcP. Conclusions: Cellular PAcP can down-regulate prostate cancer cell growth, at least partially by dephosphorylating c-ErbB-2/neu. Therefore, decreased cellular PAcP expression in cancer cells may be involved in prostate cancer progression.",
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AU - Lin, Ming-Fong

AU - Lee, Ming Shyue

AU - Zhou, Xiao Wei

AU - Andressen, John C.

AU - Meng, Tzu Ching

AU - Johansson, Sonny L.

AU - West, William W.

AU - Taylor, Rodney J.

AU - Anderson, James R.

AU - Lin, Fen Fen

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N2 - Purpose: Understanding cell proliferation regulation in hormone refractory prostate cancer may provide answers for novel solutions. Protein tyrosine phosphatases have been thought to have key roles in regulating cell proliferation and be involved in oncogenesis, although to our knowledge their functional roles in human prostate cancer remain unknown. Human prostatic acid phosphatase (PAcP), a major phosphatase in prostate epithelium, has been shown to function as a neutral protein tyrosine phosphatase in these cells. We evaluated the biological significance of cellular prostatic acid phosphatase expression in human prostate cancer cells. Materials and Methods: Immunohistochemical testing of human prostate cancer archival specimens was done to evaluate the expression of cellular PAcP. Immunoprecipitation and immunoblotting were performed to determine cellular PAcP and SH2 domain-bearing tyrosine phosphatase-1 levels as well as tyrosine phosphorylation of c-ErbB-2/neu in different human prostate cancer cells. The biological behavior of LNCaP derivative sublines was characterized in vitro and in vivo by soft agar analysis and xenograft animal inoculation. Results: Immunohistochemical staining of human prostate clearly showed that cellular levels of PAcP significantly decreases in prostate cancer cells (p<0.001). The results of biochemical characterization revealed that the cellular level of PAcP but not SHP-1, another differentiation associated protein tyrosine phosphatase, consistently correlated negatively with the growth of several human prostate cancer cell lines. Reintroducing cellular PAcP activity in prostate cancer cells by PAcP complementary DNA transfection resulted in decreased tyrosine phosphorylation of c-ErbB-2/neu, decreased proliferation rates in culture as well as decreased anchorage independent growth in soft agar. The xenograft animal model demonstrated that a higher tumor growth rate as well as larger size is associated with a lower level of cellular PAcP. Conclusions: Cellular PAcP can down-regulate prostate cancer cell growth, at least partially by dephosphorylating c-ErbB-2/neu. Therefore, decreased cellular PAcP expression in cancer cells may be involved in prostate cancer progression.

AB - Purpose: Understanding cell proliferation regulation in hormone refractory prostate cancer may provide answers for novel solutions. Protein tyrosine phosphatases have been thought to have key roles in regulating cell proliferation and be involved in oncogenesis, although to our knowledge their functional roles in human prostate cancer remain unknown. Human prostatic acid phosphatase (PAcP), a major phosphatase in prostate epithelium, has been shown to function as a neutral protein tyrosine phosphatase in these cells. We evaluated the biological significance of cellular prostatic acid phosphatase expression in human prostate cancer cells. Materials and Methods: Immunohistochemical testing of human prostate cancer archival specimens was done to evaluate the expression of cellular PAcP. Immunoprecipitation and immunoblotting were performed to determine cellular PAcP and SH2 domain-bearing tyrosine phosphatase-1 levels as well as tyrosine phosphorylation of c-ErbB-2/neu in different human prostate cancer cells. The biological behavior of LNCaP derivative sublines was characterized in vitro and in vivo by soft agar analysis and xenograft animal inoculation. Results: Immunohistochemical staining of human prostate clearly showed that cellular levels of PAcP significantly decreases in prostate cancer cells (p<0.001). The results of biochemical characterization revealed that the cellular level of PAcP but not SHP-1, another differentiation associated protein tyrosine phosphatase, consistently correlated negatively with the growth of several human prostate cancer cell lines. Reintroducing cellular PAcP activity in prostate cancer cells by PAcP complementary DNA transfection resulted in decreased tyrosine phosphorylation of c-ErbB-2/neu, decreased proliferation rates in culture as well as decreased anchorage independent growth in soft agar. The xenograft animal model demonstrated that a higher tumor growth rate as well as larger size is associated with a lower level of cellular PAcP. Conclusions: Cellular PAcP can down-regulate prostate cancer cell growth, at least partially by dephosphorylating c-ErbB-2/neu. Therefore, decreased cellular PAcP expression in cancer cells may be involved in prostate cancer progression.

KW - Disease progression

KW - Prostate

KW - Prostatic neoplasms

KW - Protein-tyrosine-phosphatase

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