Decrease in circulating hematopoietic progenitor cells by trapping in the pulmonary circulation

T. Watanabe, T. Kajiume, Y. Takaue, Y. Kawano, S. Kanamaru, S. Okamura, T. Onishi, H. Suzuya, R. Nakagawa, Y. Kuroda, James E Talmadge

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: When stem-cell grafts are infused into the venous circulation and stem/progenitor cells egress from BM, pulmonary capillary beds are the first microcirculation site that they encounter. This provides the potential for circulating progenitor cells to be trapped in the pulmonary circulation. Methods: We compared the number of progenitor cells [CD34+ cells, colony-forming unit-granulocyte-macrophage (CFU-GM), CD34+ CD41+ cells and CFU-megakaryocyte (CFU-meg)] and their expression of cell-adhesion molecules (CAM) in samples taken simultaneously from radial arteries and central veins of 21 patients following PBSC mobilization. Results: The mean (± SD) frequency of progenitor cells in the radial arteries was reduced to 79% ± 25% for CD34+ cells, 73% ± 27% for CFU-GM, 77% ± 25% for CD34+ CD41+ cells and 70% ± 29% for CFU-meg of the number in the central veins. This suggests that some progenitor cells might be trapped in the lung. No association between progenitor-cell expression of CAM and pulmonary trapping was observed. Discussion: Our data demonstrate pulmonary trapping of PBSC during mobilization, suggesting a potential inhibitory effect on PBSC harvest and medullary trafficking following graft infusion. However, the impact associated with pulmonary PBSC trapping may be negligible in the clinical setting if sufficient cells are infused.

Original languageEnglish (US)
Pages (from-to)461-466
Number of pages6
JournalCytotherapy
Volume3
Issue number6
DOIs
StatePublished - Jan 1 2001

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Pulmonary Circulation
Hematopoietic Stem Cells
Stem Cells
Lung
Granulocyte-Macrophage Progenitor Cells
Radial Artery
Megakaryocytes
Cell Adhesion Molecules
Veins
Transplants
Microcirculation

Keywords

  • Intramedullary
  • Progenitor cells
  • Trafficking
  • Trapping

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Cancer Research
  • Transplantation

Cite this

Decrease in circulating hematopoietic progenitor cells by trapping in the pulmonary circulation. / Watanabe, T.; Kajiume, T.; Takaue, Y.; Kawano, Y.; Kanamaru, S.; Okamura, S.; Onishi, T.; Suzuya, H.; Nakagawa, R.; Kuroda, Y.; Talmadge, James E.

In: Cytotherapy, Vol. 3, No. 6, 01.01.2001, p. 461-466.

Research output: Contribution to journalArticle

Watanabe, T, Kajiume, T, Takaue, Y, Kawano, Y, Kanamaru, S, Okamura, S, Onishi, T, Suzuya, H, Nakagawa, R, Kuroda, Y & Talmadge, JE 2001, 'Decrease in circulating hematopoietic progenitor cells by trapping in the pulmonary circulation', Cytotherapy, vol. 3, no. 6, pp. 461-466. https://doi.org/10.1080/146532401317248063
Watanabe T, Kajiume T, Takaue Y, Kawano Y, Kanamaru S, Okamura S et al. Decrease in circulating hematopoietic progenitor cells by trapping in the pulmonary circulation. Cytotherapy. 2001 Jan 1;3(6):461-466. https://doi.org/10.1080/146532401317248063
Watanabe, T. ; Kajiume, T. ; Takaue, Y. ; Kawano, Y. ; Kanamaru, S. ; Okamura, S. ; Onishi, T. ; Suzuya, H. ; Nakagawa, R. ; Kuroda, Y. ; Talmadge, James E. / Decrease in circulating hematopoietic progenitor cells by trapping in the pulmonary circulation. In: Cytotherapy. 2001 ; Vol. 3, No. 6. pp. 461-466.
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AU - Okamura, S.

AU - Onishi, T.

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AB - Background: When stem-cell grafts are infused into the venous circulation and stem/progenitor cells egress from BM, pulmonary capillary beds are the first microcirculation site that they encounter. This provides the potential for circulating progenitor cells to be trapped in the pulmonary circulation. Methods: We compared the number of progenitor cells [CD34+ cells, colony-forming unit-granulocyte-macrophage (CFU-GM), CD34+ CD41+ cells and CFU-megakaryocyte (CFU-meg)] and their expression of cell-adhesion molecules (CAM) in samples taken simultaneously from radial arteries and central veins of 21 patients following PBSC mobilization. Results: The mean (± SD) frequency of progenitor cells in the radial arteries was reduced to 79% ± 25% for CD34+ cells, 73% ± 27% for CFU-GM, 77% ± 25% for CD34+ CD41+ cells and 70% ± 29% for CFU-meg of the number in the central veins. This suggests that some progenitor cells might be trapped in the lung. No association between progenitor-cell expression of CAM and pulmonary trapping was observed. Discussion: Our data demonstrate pulmonary trapping of PBSC during mobilization, suggesting a potential inhibitory effect on PBSC harvest and medullary trafficking following graft infusion. However, the impact associated with pulmonary PBSC trapping may be negligible in the clinical setting if sufficient cells are infused.

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