Decontamination of a hospital room using gaseous chlorine dioxide: Bacillus anthracis, francisella tularensis, and Yersinia pestis

John-Martin J Lowe, Shawn G. Gibbs, Peter Charles Iwen, Philip W. Smith, Angela L Hewlett

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This study assessed the efficacy of gaseous chlorine dioxide for inactivation of Bacillus anthracis, Francisella tularensis, and Yersinia pestis in a hospital patient care suite. Spore and vegetative cells of Bacillus anthracis Sterne 34F2, spores of Bacillus atrophaeus ATCC 9372 and vegetative cells of both Francisella tularensis ATCC 6223 and Yersinia pestis A1122 were exposed to gaseous chlorine dioxide in a patient care suite. Organism inactivation was then assessed by log reduction in viable organisms postexposure to chlorine dioxide gas compared to non-exposed control organism. Hospital room decontamination protocols utilizing chlorine dioxide gas concentrations of 377 to 385 ppm maintained to exposures of 767 ppm-hours with 65% relative humidity consistently achieved complete inactivation of B. anthracis and B. at-rophaeus spores, as well as vegetative cells of B. anthracis, F. tularensis, and Y. pestis. Decrease in exposure (ppm-hours) and relative humidity (<65%) or restricting airflow reduced inactivation but achieved >8 log reductions in organisms. Up to 10-log reductions were achieved in a hospital room with limited impact on adjacent areas, indicating chlorine dioxide concentrations needed for decontamination of highly concentrated (>6 logs) organisms can be achieved throughout a hospital room. This study translates laboratory chlorine dioxide fumigation studies applied in a complex clinical environment.

Original languageEnglish (US)
Pages (from-to)533-539
Number of pages7
JournalJournal of occupational and environmental hygiene
Volume10
Issue number10
DOIs
StatePublished - Sep 26 2013

Fingerprint

Francisella tularensis
Yersinia pestis
Bacillus anthracis
Decontamination
Spores
Humidity
Patient Care
Gases
Fumigation
Bacillus
chlorine dioxide

Keywords

  • Bacillus anthracis
  • Chlorine dioxide
  • Decontamination
  • Francisella tularen-sis
  • Yersinia pestis

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

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abstract = "This study assessed the efficacy of gaseous chlorine dioxide for inactivation of Bacillus anthracis, Francisella tularensis, and Yersinia pestis in a hospital patient care suite. Spore and vegetative cells of Bacillus anthracis Sterne 34F2, spores of Bacillus atrophaeus ATCC 9372 and vegetative cells of both Francisella tularensis ATCC 6223 and Yersinia pestis A1122 were exposed to gaseous chlorine dioxide in a patient care suite. Organism inactivation was then assessed by log reduction in viable organisms postexposure to chlorine dioxide gas compared to non-exposed control organism. Hospital room decontamination protocols utilizing chlorine dioxide gas concentrations of 377 to 385 ppm maintained to exposures of 767 ppm-hours with 65{\%} relative humidity consistently achieved complete inactivation of B. anthracis and B. at-rophaeus spores, as well as vegetative cells of B. anthracis, F. tularensis, and Y. pestis. Decrease in exposure (ppm-hours) and relative humidity (<65{\%}) or restricting airflow reduced inactivation but achieved >8 log reductions in organisms. Up to 10-log reductions were achieved in a hospital room with limited impact on adjacent areas, indicating chlorine dioxide concentrations needed for decontamination of highly concentrated (>6 logs) organisms can be achieved throughout a hospital room. This study translates laboratory chlorine dioxide fumigation studies applied in a complex clinical environment.",
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