Deciphering molecular details in the assembly of alpha-type carboxysome

Yilan Liu, Xinyuan He, Weiping Lim, Joshua Mueller, Justin Lawrie, Levi Kramer, Jiantao Guo, Wei Niu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Bacterial microcompartments (BMCs) are promising natural protein structures for applications that require the segregation of certain metabolic functions or molecular species in a defined microenvironment. To understand how endogenous cargos are packaged inside the protein shell is key for using BMCs as nano-scale reactors or delivery vesicles. In this report, we studied the encapsulation of RuBisCO into the α-type carboxysome from Halothiobacillus neapolitan. Our experimental data revealed that the CsoS2 scaffold proteins engage RuBisCO enzyme through an interaction with the small subunit (CbbS). In addition, the N domain of the large subunit (CbbL) of RuBisCO interacts with all shell proteins that can form the hexamers. The binding affinity between the N domain of CbbL and one of the major shell proteins, CsoS1C, is within the submicromolar range. The absence of the N domain also prevented the encapsulation of the rest of the RuBisCO subunits. Our findings complete the picture of how RuBisCOs are encapsulated into the α-type carboxysome and provide insights for future studies and engineering of carboxysome as a protein shell.

Original languageEnglish (US)
Article number15062
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Proteins
Halothiobacillus
Enzymes

ASJC Scopus subject areas

  • General

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Deciphering molecular details in the assembly of alpha-type carboxysome. / Liu, Yilan; He, Xinyuan; Lim, Weiping; Mueller, Joshua; Lawrie, Justin; Kramer, Levi; Guo, Jiantao; Niu, Wei.

In: Scientific reports, Vol. 8, No. 1, 15062, 01.12.2018.

Research output: Contribution to journalArticle

Liu, Yilan ; He, Xinyuan ; Lim, Weiping ; Mueller, Joshua ; Lawrie, Justin ; Kramer, Levi ; Guo, Jiantao ; Niu, Wei. / Deciphering molecular details in the assembly of alpha-type carboxysome. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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