DAWDLE interacts with DICER-LIKE proteins to mediate small RNA biogenesis

Shuxin Zhang, Yongchao Dou, Shengjun Li, Guodong Ren, David Chevalier, Chi Zhang, Bin Yu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

DAWDLE (DDL) is a conserved forkhead-associated (FHA) domain-containing protein with essential roles in plant development and immunity. It acts in the biogenesis of microRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs), which regulate gene expression at the transcriptional and/or posttranscriptional levels. However, the functional mechanism of DDL and its impact on exogenous siRNAs remain elusive. Here, we report that DDL is required for the biogenesis of siRNAs derived from sense transgenes and inverted-repeat transgenes. Furthermore, we show that a mutation in the FHA domain of DDL disrupts the interaction of DDL with DICER-LIKE1 (DCL1), which is the enzyme that catalyzes miRNA maturation from primary miRNA transcripts (pri-miRNAs), resulting in impaired pri-miRNA processing. Moreover, we demonstrate that DDL interacts with DCL3, which is a DCL1 homolog responsible for siRNA production, and this interaction is crucial for optimal DCL3 activity. These results reveal that the interaction of DDL with DCLs is required for the biogenesis of miRNAs and siRNAs in Arabidopsis (Arabidopsis thaliana).

Original languageEnglish (US)
Pages (from-to)1142-1151
Number of pages10
JournalPlant physiology
Volume177
Issue number3
DOIs
StatePublished - Jun 2018

Fingerprint

small interfering RNA
MicroRNAs
microRNA
Small Interfering RNA
RNA
Proteins
Transgenes
Arabidopsis
proteins
transgenes
Plant Immunity
Plant Development
plant development
Arabidopsis thaliana
immunity
biogenesis
mutation
Gene Expression
gene expression
Mutation

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

DAWDLE interacts with DICER-LIKE proteins to mediate small RNA biogenesis. / Zhang, Shuxin; Dou, Yongchao; Li, Shengjun; Ren, Guodong; Chevalier, David; Zhang, Chi; Yu, Bin.

In: Plant physiology, Vol. 177, No. 3, 06.2018, p. 1142-1151.

Research output: Contribution to journalArticle

Zhang, Shuxin ; Dou, Yongchao ; Li, Shengjun ; Ren, Guodong ; Chevalier, David ; Zhang, Chi ; Yu, Bin. / DAWDLE interacts with DICER-LIKE proteins to mediate small RNA biogenesis. In: Plant physiology. 2018 ; Vol. 177, No. 3. pp. 1142-1151.
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