Cytologic features in vitreous preparations of patients with suspicion of intraocular lymphoma

Erika F. Rodriguez, Yasir J. Sepah, Hyun Soo Jang, Mohamed Ibrahim, Quan Dong Nguyen, Fausto J. Rodriguez

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Intraocular lymphoma may occur, primarily with or without overt parenchymal CNS lymphoma or secondarily from a variety of other lymphomas. The diagnosis is frequently based on cytologic features and/or a limited panel of ancillary techniques. Twenty-seven cytology samples from the vitreous preparations of 20 patients with clinical suspicion or histopathologic diagnoses of lymphoma were retrospectively reviewed (2007-2011). Floaters and decreased visual acuity were the most common ocular symptoms. Sixteen (of 20) patients had at least one cytology that was "positive" or "suspicious" for lymphoma (F = 10; M = 6, median age = 71 years, range = 52-82). Involvement was limited to the eye in half of the cases (n = 8). Seven patients had CNS involvement, and three had systemic lymphoma. Cytologic specimens were classified as large B cell lymphoma (LBCL) (n = 13), suspicious for LBCL (n = 5), atypical/negative (n = 5), and consistent with NK-T cell lymphoma (n = 1). Three cytologic samples were acellular. Cytologic findings included increased cellularity (high n = 7, moderate n = 7), large to medium (n = 19) cell size, marked nuclear irregularities (n = 12), frequent apoptosis (n = 7), lymphoglandular bodies (n = 12), and necrosis (n = 12). Easily identifiable mitotic figures were present in two cases. CD20 immunocytochemistry performed in 15 cases with available cell blocks/destained cytospins preparations, was confirmatory in 8 cases. Cytologic evaluation of intraocular lymphoma is possible in vitreous specimens. Extraocular involvement was not present in half of the cases. The most useful cytologic features include increased cellularity, necrosis, and nuclear enlargement. Ancillary immunocytochemical studies are useful and support the morphologic impression in a subset of cases.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalDiagnostic cytopathology
Volume42
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

Intraocular Lymphoma
Lymphoma
B-Cell Lymphoma
Cell Biology
Necrosis
Cell Nucleus Size
T-Cell Lymphoma
Natural Killer Cells
Visual Acuity
Immunohistochemistry
Apoptosis

Keywords

  • CNS lymphoma
  • floaters
  • intraocular lymphoma
  • lymphoma
  • optical coherence tomography
  • vitreous cytology
  • vitritis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Rodriguez, E. F., Sepah, Y. J., Jang, H. S., Ibrahim, M., Nguyen, Q. D., & Rodriguez, F. J. (2014). Cytologic features in vitreous preparations of patients with suspicion of intraocular lymphoma. Diagnostic cytopathology, 42(1), 37-44. https://doi.org/10.1002/dc.23059

Cytologic features in vitreous preparations of patients with suspicion of intraocular lymphoma. / Rodriguez, Erika F.; Sepah, Yasir J.; Jang, Hyun Soo; Ibrahim, Mohamed; Nguyen, Quan Dong; Rodriguez, Fausto J.

In: Diagnostic cytopathology, Vol. 42, No. 1, 01.01.2014, p. 37-44.

Research output: Contribution to journalArticle

Rodriguez, EF, Sepah, YJ, Jang, HS, Ibrahim, M, Nguyen, QD & Rodriguez, FJ 2014, 'Cytologic features in vitreous preparations of patients with suspicion of intraocular lymphoma', Diagnostic cytopathology, vol. 42, no. 1, pp. 37-44. https://doi.org/10.1002/dc.23059
Rodriguez, Erika F. ; Sepah, Yasir J. ; Jang, Hyun Soo ; Ibrahim, Mohamed ; Nguyen, Quan Dong ; Rodriguez, Fausto J. / Cytologic features in vitreous preparations of patients with suspicion of intraocular lymphoma. In: Diagnostic cytopathology. 2014 ; Vol. 42, No. 1. pp. 37-44.
@article{499f5d55e8374d66a7c32555e908c2b3,
title = "Cytologic features in vitreous preparations of patients with suspicion of intraocular lymphoma",
abstract = "Intraocular lymphoma may occur, primarily with or without overt parenchymal CNS lymphoma or secondarily from a variety of other lymphomas. The diagnosis is frequently based on cytologic features and/or a limited panel of ancillary techniques. Twenty-seven cytology samples from the vitreous preparations of 20 patients with clinical suspicion or histopathologic diagnoses of lymphoma were retrospectively reviewed (2007-2011). Floaters and decreased visual acuity were the most common ocular symptoms. Sixteen (of 20) patients had at least one cytology that was {"}positive{"} or {"}suspicious{"} for lymphoma (F = 10; M = 6, median age = 71 years, range = 52-82). Involvement was limited to the eye in half of the cases (n = 8). Seven patients had CNS involvement, and three had systemic lymphoma. Cytologic specimens were classified as large B cell lymphoma (LBCL) (n = 13), suspicious for LBCL (n = 5), atypical/negative (n = 5), and consistent with NK-T cell lymphoma (n = 1). Three cytologic samples were acellular. Cytologic findings included increased cellularity (high n = 7, moderate n = 7), large to medium (n = 19) cell size, marked nuclear irregularities (n = 12), frequent apoptosis (n = 7), lymphoglandular bodies (n = 12), and necrosis (n = 12). Easily identifiable mitotic figures were present in two cases. CD20 immunocytochemistry performed in 15 cases with available cell blocks/destained cytospins preparations, was confirmatory in 8 cases. Cytologic evaluation of intraocular lymphoma is possible in vitreous specimens. Extraocular involvement was not present in half of the cases. The most useful cytologic features include increased cellularity, necrosis, and nuclear enlargement. Ancillary immunocytochemical studies are useful and support the morphologic impression in a subset of cases.",
keywords = "CNS lymphoma, floaters, intraocular lymphoma, lymphoma, optical coherence tomography, vitreous cytology, vitritis",
author = "Rodriguez, {Erika F.} and Sepah, {Yasir J.} and Jang, {Hyun Soo} and Mohamed Ibrahim and Nguyen, {Quan Dong} and Rodriguez, {Fausto J.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1002/dc.23059",
language = "English (US)",
volume = "42",
pages = "37--44",
journal = "Diagnostic Cytopathology",
issn = "8755-1039",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Cytologic features in vitreous preparations of patients with suspicion of intraocular lymphoma

AU - Rodriguez, Erika F.

AU - Sepah, Yasir J.

AU - Jang, Hyun Soo

AU - Ibrahim, Mohamed

AU - Nguyen, Quan Dong

AU - Rodriguez, Fausto J.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Intraocular lymphoma may occur, primarily with or without overt parenchymal CNS lymphoma or secondarily from a variety of other lymphomas. The diagnosis is frequently based on cytologic features and/or a limited panel of ancillary techniques. Twenty-seven cytology samples from the vitreous preparations of 20 patients with clinical suspicion or histopathologic diagnoses of lymphoma were retrospectively reviewed (2007-2011). Floaters and decreased visual acuity were the most common ocular symptoms. Sixteen (of 20) patients had at least one cytology that was "positive" or "suspicious" for lymphoma (F = 10; M = 6, median age = 71 years, range = 52-82). Involvement was limited to the eye in half of the cases (n = 8). Seven patients had CNS involvement, and three had systemic lymphoma. Cytologic specimens were classified as large B cell lymphoma (LBCL) (n = 13), suspicious for LBCL (n = 5), atypical/negative (n = 5), and consistent with NK-T cell lymphoma (n = 1). Three cytologic samples were acellular. Cytologic findings included increased cellularity (high n = 7, moderate n = 7), large to medium (n = 19) cell size, marked nuclear irregularities (n = 12), frequent apoptosis (n = 7), lymphoglandular bodies (n = 12), and necrosis (n = 12). Easily identifiable mitotic figures were present in two cases. CD20 immunocytochemistry performed in 15 cases with available cell blocks/destained cytospins preparations, was confirmatory in 8 cases. Cytologic evaluation of intraocular lymphoma is possible in vitreous specimens. Extraocular involvement was not present in half of the cases. The most useful cytologic features include increased cellularity, necrosis, and nuclear enlargement. Ancillary immunocytochemical studies are useful and support the morphologic impression in a subset of cases.

AB - Intraocular lymphoma may occur, primarily with or without overt parenchymal CNS lymphoma or secondarily from a variety of other lymphomas. The diagnosis is frequently based on cytologic features and/or a limited panel of ancillary techniques. Twenty-seven cytology samples from the vitreous preparations of 20 patients with clinical suspicion or histopathologic diagnoses of lymphoma were retrospectively reviewed (2007-2011). Floaters and decreased visual acuity were the most common ocular symptoms. Sixteen (of 20) patients had at least one cytology that was "positive" or "suspicious" for lymphoma (F = 10; M = 6, median age = 71 years, range = 52-82). Involvement was limited to the eye in half of the cases (n = 8). Seven patients had CNS involvement, and three had systemic lymphoma. Cytologic specimens were classified as large B cell lymphoma (LBCL) (n = 13), suspicious for LBCL (n = 5), atypical/negative (n = 5), and consistent with NK-T cell lymphoma (n = 1). Three cytologic samples were acellular. Cytologic findings included increased cellularity (high n = 7, moderate n = 7), large to medium (n = 19) cell size, marked nuclear irregularities (n = 12), frequent apoptosis (n = 7), lymphoglandular bodies (n = 12), and necrosis (n = 12). Easily identifiable mitotic figures were present in two cases. CD20 immunocytochemistry performed in 15 cases with available cell blocks/destained cytospins preparations, was confirmatory in 8 cases. Cytologic evaluation of intraocular lymphoma is possible in vitreous specimens. Extraocular involvement was not present in half of the cases. The most useful cytologic features include increased cellularity, necrosis, and nuclear enlargement. Ancillary immunocytochemical studies are useful and support the morphologic impression in a subset of cases.

KW - CNS lymphoma

KW - floaters

KW - intraocular lymphoma

KW - lymphoma

KW - optical coherence tomography

KW - vitreous cytology

KW - vitritis

UR - http://www.scopus.com/inward/record.url?scp=84891477830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891477830&partnerID=8YFLogxK

U2 - 10.1002/dc.23059

DO - 10.1002/dc.23059

M3 - Article

C2 - 24167062

AN - SCOPUS:84891477830

VL - 42

SP - 37

EP - 44

JO - Diagnostic Cytopathology

JF - Diagnostic Cytopathology

SN - 8755-1039

IS - 1

ER -