Cytokine pattern in aneurysmal and occlusive disease of the aorta

Valerie A. Davis, Raisa N. Persidskaia, Lisa M. Baca-Regen, Nicola Fiotti, Brian G. Halloran, Bernard Timothy Baxter

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background. Prominent inflammatory infiltrates of macrophages and T-lymphocytes are found in both aortic occlusive disease (AOD) and abdominal aortic aneurysms (AAA). These cells secrete different cytokines that might affect matrix turnover through modulation of matrix metalloproteinase expression. A different cytokine pattern might account for the evolution of AOD vs AAA. Materials and Methods. Six different cytokines were examined to determine whether AOD and AAA could be characterized by unique cytokine patterns. AOD (n = 8) and AAA (n = 8) tissues were collected and serially treated with salt, dimethyl sulfoxide, and urea buffers to extract the soluble matrix or cell-bound cytokines. Levels of IL-1β, TNF-α, IL-10, IL-12, and IFN-γ were measured by immunoenzymatic methods. Additionally, RNA levels of IL-12 and IFN-γ were measured. Results. AAA tissue contained higher levels of IL-10 compared to AOD tissue (P < 0.05). Higher levels of the proinflammatory cytokines IL-1β, TNF-α, and IL-6 were found in AOD (P < 0.05). mRNA levels of IL-12 and IFN-γ did not differ between the diseases. Aortic tissues contained large amounts of matrix or cell-bound cytokines. Conclusions. AAA is characterized by greater levels of IL-10 while IL-1β, TNF-α, and IL-6 are higher in AOD. Targeted deletion of these cytokines in animal models might help in identifying their role in the progression of AAA.

Original languageEnglish (US)
Pages (from-to)152-156
Number of pages5
JournalJournal of Surgical Research
Volume101
Issue number2
DOIs
StatePublished - Jan 1 2001

Fingerprint

Aortic Diseases
Abdominal Aortic Aneurysm
Aorta
Cytokines
Interleukin-12
Interleukin-1
Interleukin-10
Interleukin-6
Dimethyl Sulfoxide
Matrix Metalloproteinases
Urea
Buffers
Animal Models
Salts
Macrophages
RNA
T-Lymphocytes
Messenger RNA

Keywords

  • Aneurysm
  • Aorta
  • Atherosclerosis
  • Cytokines
  • IL- 1β
  • IL-10
  • IL-6
  • Inflammation
  • Lymphocytes
  • TNF-α

ASJC Scopus subject areas

  • Surgery

Cite this

Davis, V. A., Persidskaia, R. N., Baca-Regen, L. M., Fiotti, N., Halloran, B. G., & Baxter, B. T. (2001). Cytokine pattern in aneurysmal and occlusive disease of the aorta. Journal of Surgical Research, 101(2), 152-156. https://doi.org/10.1006/jsre.2001.6281

Cytokine pattern in aneurysmal and occlusive disease of the aorta. / Davis, Valerie A.; Persidskaia, Raisa N.; Baca-Regen, Lisa M.; Fiotti, Nicola; Halloran, Brian G.; Baxter, Bernard Timothy.

In: Journal of Surgical Research, Vol. 101, No. 2, 01.01.2001, p. 152-156.

Research output: Contribution to journalArticle

Davis, VA, Persidskaia, RN, Baca-Regen, LM, Fiotti, N, Halloran, BG & Baxter, BT 2001, 'Cytokine pattern in aneurysmal and occlusive disease of the aorta', Journal of Surgical Research, vol. 101, no. 2, pp. 152-156. https://doi.org/10.1006/jsre.2001.6281
Davis VA, Persidskaia RN, Baca-Regen LM, Fiotti N, Halloran BG, Baxter BT. Cytokine pattern in aneurysmal and occlusive disease of the aorta. Journal of Surgical Research. 2001 Jan 1;101(2):152-156. https://doi.org/10.1006/jsre.2001.6281
Davis, Valerie A. ; Persidskaia, Raisa N. ; Baca-Regen, Lisa M. ; Fiotti, Nicola ; Halloran, Brian G. ; Baxter, Bernard Timothy. / Cytokine pattern in aneurysmal and occlusive disease of the aorta. In: Journal of Surgical Research. 2001 ; Vol. 101, No. 2. pp. 152-156.
@article{b867b915e2754d279558f68e6a1173b7,
title = "Cytokine pattern in aneurysmal and occlusive disease of the aorta",
abstract = "Background. Prominent inflammatory infiltrates of macrophages and T-lymphocytes are found in both aortic occlusive disease (AOD) and abdominal aortic aneurysms (AAA). These cells secrete different cytokines that might affect matrix turnover through modulation of matrix metalloproteinase expression. A different cytokine pattern might account for the evolution of AOD vs AAA. Materials and Methods. Six different cytokines were examined to determine whether AOD and AAA could be characterized by unique cytokine patterns. AOD (n = 8) and AAA (n = 8) tissues were collected and serially treated with salt, dimethyl sulfoxide, and urea buffers to extract the soluble matrix or cell-bound cytokines. Levels of IL-1β, TNF-α, IL-10, IL-12, and IFN-γ were measured by immunoenzymatic methods. Additionally, RNA levels of IL-12 and IFN-γ were measured. Results. AAA tissue contained higher levels of IL-10 compared to AOD tissue (P < 0.05). Higher levels of the proinflammatory cytokines IL-1β, TNF-α, and IL-6 were found in AOD (P < 0.05). mRNA levels of IL-12 and IFN-γ did not differ between the diseases. Aortic tissues contained large amounts of matrix or cell-bound cytokines. Conclusions. AAA is characterized by greater levels of IL-10 while IL-1β, TNF-α, and IL-6 are higher in AOD. Targeted deletion of these cytokines in animal models might help in identifying their role in the progression of AAA.",
keywords = "Aneurysm, Aorta, Atherosclerosis, Cytokines, IL- 1β, IL-10, IL-6, Inflammation, Lymphocytes, TNF-α",
author = "Davis, {Valerie A.} and Persidskaia, {Raisa N.} and Baca-Regen, {Lisa M.} and Nicola Fiotti and Halloran, {Brian G.} and Baxter, {Bernard Timothy}",
year = "2001",
month = "1",
day = "1",
doi = "10.1006/jsre.2001.6281",
language = "English (US)",
volume = "101",
pages = "152--156",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Cytokine pattern in aneurysmal and occlusive disease of the aorta

AU - Davis, Valerie A.

AU - Persidskaia, Raisa N.

AU - Baca-Regen, Lisa M.

AU - Fiotti, Nicola

AU - Halloran, Brian G.

AU - Baxter, Bernard Timothy

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Background. Prominent inflammatory infiltrates of macrophages and T-lymphocytes are found in both aortic occlusive disease (AOD) and abdominal aortic aneurysms (AAA). These cells secrete different cytokines that might affect matrix turnover through modulation of matrix metalloproteinase expression. A different cytokine pattern might account for the evolution of AOD vs AAA. Materials and Methods. Six different cytokines were examined to determine whether AOD and AAA could be characterized by unique cytokine patterns. AOD (n = 8) and AAA (n = 8) tissues were collected and serially treated with salt, dimethyl sulfoxide, and urea buffers to extract the soluble matrix or cell-bound cytokines. Levels of IL-1β, TNF-α, IL-10, IL-12, and IFN-γ were measured by immunoenzymatic methods. Additionally, RNA levels of IL-12 and IFN-γ were measured. Results. AAA tissue contained higher levels of IL-10 compared to AOD tissue (P < 0.05). Higher levels of the proinflammatory cytokines IL-1β, TNF-α, and IL-6 were found in AOD (P < 0.05). mRNA levels of IL-12 and IFN-γ did not differ between the diseases. Aortic tissues contained large amounts of matrix or cell-bound cytokines. Conclusions. AAA is characterized by greater levels of IL-10 while IL-1β, TNF-α, and IL-6 are higher in AOD. Targeted deletion of these cytokines in animal models might help in identifying their role in the progression of AAA.

AB - Background. Prominent inflammatory infiltrates of macrophages and T-lymphocytes are found in both aortic occlusive disease (AOD) and abdominal aortic aneurysms (AAA). These cells secrete different cytokines that might affect matrix turnover through modulation of matrix metalloproteinase expression. A different cytokine pattern might account for the evolution of AOD vs AAA. Materials and Methods. Six different cytokines were examined to determine whether AOD and AAA could be characterized by unique cytokine patterns. AOD (n = 8) and AAA (n = 8) tissues were collected and serially treated with salt, dimethyl sulfoxide, and urea buffers to extract the soluble matrix or cell-bound cytokines. Levels of IL-1β, TNF-α, IL-10, IL-12, and IFN-γ were measured by immunoenzymatic methods. Additionally, RNA levels of IL-12 and IFN-γ were measured. Results. AAA tissue contained higher levels of IL-10 compared to AOD tissue (P < 0.05). Higher levels of the proinflammatory cytokines IL-1β, TNF-α, and IL-6 were found in AOD (P < 0.05). mRNA levels of IL-12 and IFN-γ did not differ between the diseases. Aortic tissues contained large amounts of matrix or cell-bound cytokines. Conclusions. AAA is characterized by greater levels of IL-10 while IL-1β, TNF-α, and IL-6 are higher in AOD. Targeted deletion of these cytokines in animal models might help in identifying their role in the progression of AAA.

KW - Aneurysm

KW - Aorta

KW - Atherosclerosis

KW - Cytokines

KW - IL- 1β

KW - IL-10

KW - IL-6

KW - Inflammation

KW - Lymphocytes

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=0035678227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035678227&partnerID=8YFLogxK

U2 - 10.1006/jsre.2001.6281

DO - 10.1006/jsre.2001.6281

M3 - Article

VL - 101

SP - 152

EP - 156

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -