Cytogenetic abnormalities in colon cancer patients: A comparison of T- and B-Lymphocytes

B. J. Dave, V. L. Hopwood, J. I. Hughes, G. L. Jackson, D. Melillo, S. Pathak

Research output: Contribution to journalArticle

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Abstract

Cytogenetic analysis of peripheral blood lymphocytes of 19 colorectal cancer patients was carried out in short term blood cultures (T-cells) as well as in Epstein-Barr virus transformed B-cell lymphoblastoid cell lines. One hundred Giemsa-banded metaphases from the T lymphocytes and 50 metaphases from the B lymphocytes of each patient were evaluated for cytogenetic abnormalities. Clonality was not observed in every paired sample. Structural and/or numerical aberrations were most frequent in chromosomes no 1, no2, no5, no7, no9, no12, no14, no17, no18 and no21. Aberrations among these chromosomes could be observed individually in either of the cultures, which proves that the analysis of both cultures (T and B cells) is complementary to each other. In some cases involving multiple primary cancers it was interesting that the specific chromosomal change, crucial for a particular malignancy, was identified only in the lymphoblastoid cell line analysis. This supports the notion that B-cell analysis can serve as a useful adjunct to the study of short-term blood cultures and also poses a question as to whether the specific chromosomal changes observed in the analysis are confined to the B-cell lineage.

Original languageEnglish (US)
Pages (from-to)433-438
Number of pages6
JournalAnticancer Research
Volume13
Issue number2
StatePublished - Jan 1 1993

Fingerprint

Chromosome Aberrations
Colonic Neoplasms
B-Lymphocytes
Metaphase
T-Lymphocytes
Cell Line
Chromosomes, Human, Pair 1
Cytogenetic Analysis
Cell Lineage
Human Herpesvirus 4
Colorectal Neoplasms
Neoplasms
Lymphocytes
Blood Culture

Keywords

  • Colorectal cancer
  • Cytogenetic abnormalities
  • Epstein-Barr virus
  • Hereditary factors
  • Lymphoblastoid
  • Malignancy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Dave, B. J., Hopwood, V. L., Hughes, J. I., Jackson, G. L., Melillo, D., & Pathak, S. (1993). Cytogenetic abnormalities in colon cancer patients: A comparison of T- and B-Lymphocytes. Anticancer Research, 13(2), 433-438.

Cytogenetic abnormalities in colon cancer patients : A comparison of T- and B-Lymphocytes. / Dave, B. J.; Hopwood, V. L.; Hughes, J. I.; Jackson, G. L.; Melillo, D.; Pathak, S.

In: Anticancer Research, Vol. 13, No. 2, 01.01.1993, p. 433-438.

Research output: Contribution to journalArticle

Dave, BJ, Hopwood, VL, Hughes, JI, Jackson, GL, Melillo, D & Pathak, S 1993, 'Cytogenetic abnormalities in colon cancer patients: A comparison of T- and B-Lymphocytes', Anticancer Research, vol. 13, no. 2, pp. 433-438.
Dave BJ, Hopwood VL, Hughes JI, Jackson GL, Melillo D, Pathak S. Cytogenetic abnormalities in colon cancer patients: A comparison of T- and B-Lymphocytes. Anticancer Research. 1993 Jan 1;13(2):433-438.
Dave, B. J. ; Hopwood, V. L. ; Hughes, J. I. ; Jackson, G. L. ; Melillo, D. ; Pathak, S. / Cytogenetic abnormalities in colon cancer patients : A comparison of T- and B-Lymphocytes. In: Anticancer Research. 1993 ; Vol. 13, No. 2. pp. 433-438.
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