Cystic fibrosis: molecular weight estimation of the ciliary inhibitor

D. R. Barnett, A. Kurosky, B. H. Bowman, H. T. Hutchison, I. Schmoyer, S. D. Carson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The molecular weight of the ciliary inhibitor fractionated from media of cultured fibroblasts derived from cystic fibrosis patients was estimated by sucrose density gradient ultracentrifugation, membrane filtration and gel filtration. Ultracentrifugation analysis indicated a molecular weight closer to the α 1 chain of haptoglobin (9,100) than to immunoglobulin G (150,000). Molecular filtration on Amicon membranes indicated a molecular weight range of 1,000 to 10,000. Gel filtration on Sephadex G 50F indicated a molecular weight of 4,500 to 10,000. These results characterize the ciliary inhibitor from fibroblast media as a small molecular weight protein.

Original languageEnglish (US)
Pages (from-to)703-708
Number of pages6
JournalTexas Reports on Biology and Medicine
Volume31
Issue number4
StatePublished - Dec 1 1973

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Cystic Fibrosis
Molecular Weight
Ultracentrifugation
Gel Chromatography
Fibroblasts
Haptoglobins
Membranes
Sucrose
Immunoglobulin G
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Barnett, D. R., Kurosky, A., Bowman, B. H., Hutchison, H. T., Schmoyer, I., & Carson, S. D. (1973). Cystic fibrosis: molecular weight estimation of the ciliary inhibitor. Texas Reports on Biology and Medicine, 31(4), 703-708.

Cystic fibrosis : molecular weight estimation of the ciliary inhibitor. / Barnett, D. R.; Kurosky, A.; Bowman, B. H.; Hutchison, H. T.; Schmoyer, I.; Carson, S. D.

In: Texas Reports on Biology and Medicine, Vol. 31, No. 4, 01.12.1973, p. 703-708.

Research output: Contribution to journalArticle

Barnett, DR, Kurosky, A, Bowman, BH, Hutchison, HT, Schmoyer, I & Carson, SD 1973, 'Cystic fibrosis: molecular weight estimation of the ciliary inhibitor', Texas Reports on Biology and Medicine, vol. 31, no. 4, pp. 703-708.
Barnett DR, Kurosky A, Bowman BH, Hutchison HT, Schmoyer I, Carson SD. Cystic fibrosis: molecular weight estimation of the ciliary inhibitor. Texas Reports on Biology and Medicine. 1973 Dec 1;31(4):703-708.
Barnett, D. R. ; Kurosky, A. ; Bowman, B. H. ; Hutchison, H. T. ; Schmoyer, I. ; Carson, S. D. / Cystic fibrosis : molecular weight estimation of the ciliary inhibitor. In: Texas Reports on Biology and Medicine. 1973 ; Vol. 31, No. 4. pp. 703-708.
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