Cyclophilins are encoded by a small gene family in rice

Wallace G Buchholz, Larry Harris-Haller, Richard T. DeRose, Timothy C. Hall

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1 and Cyp2 have seven extra amino acid residues in the N-terminal portion of the proteins that are not found in human or other non-plant cyclophilins, suggesting that this is a characteristic of plant cyclophilins. Cyp2 was expressed as 1000 nt transcripts in leaf and root tissues. Cyp1 was expressed as 800 and 900 nt transcripts. Whereas the 900 nt transcript was present in both root and leaf mRNA, the 800 nt transcript was only detectable in root mRNA. A genomic clone of Cyp2 was isolated, sequenced and shown to lack introns. A single transcriptional start site was identified 27 residues downstream of a putative TATA box. The 5′ end of the transcript was shown to contain a region rich in adenyl residues (27 of 35). This region would not be conducive to secondary structure formation, which raises the possibility that Cyp2 might be preferentially translated during stress conditions.

Original languageEnglish (US)
Pages (from-to)837-843
Number of pages7
JournalPlant Molecular Biology
Volume25
Issue number5
DOIs
StatePublished - Aug 1 1994

Fingerprint

cyclophilins
Cyclophilins
Genes
rice
genes
Clone Cells
clones
genomics
Amino Acids
TATA box
Messenger RNA
TATA Box
T-cells
plant characteristics
Introns
introns
leaves
Amino Acid Sequence
amino acid sequences
Complementary DNA

Keywords

  • Oryza sativa
  • cyclophilin
  • peptidyl-prolyl cis-trans isomerase

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Cyclophilins are encoded by a small gene family in rice. / Buchholz, Wallace G; Harris-Haller, Larry; DeRose, Richard T.; Hall, Timothy C.

In: Plant Molecular Biology, Vol. 25, No. 5, 01.08.1994, p. 837-843.

Research output: Contribution to journalArticle

Buchholz, WG, Harris-Haller, L, DeRose, RT & Hall, TC 1994, 'Cyclophilins are encoded by a small gene family in rice', Plant Molecular Biology, vol. 25, no. 5, pp. 837-843. https://doi.org/10.1007/BF00028878
Buchholz, Wallace G ; Harris-Haller, Larry ; DeRose, Richard T. ; Hall, Timothy C. / Cyclophilins are encoded by a small gene family in rice. In: Plant Molecular Biology. 1994 ; Vol. 25, No. 5. pp. 837-843.
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