Cyclin D1 abnormalities and tobacco exposure in head and neck squamous cell carcinoma

Bruce J. Davidson, William Michael Lydiatt, Megan P. Abate, Stimson P. Schantz, R. S.K. Chaganti

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background. Amplification of the cyclin D1 (CCND1) gene, which encodes a cell cycle regulating protein, has been described in several solid tumors including head and neck squamous cell carcinoma (HNSCC). While correlations between CCND1 amplification and tumor behavior have been suggested, no investigation has focused on risk factor exposure as a potential cause of CCND1 alteration. Methods: Southern blotting was used to identify CCND1 amplification in 57 previously untreated HNSCC tumor specimens. Tissue from 27 cases was analyzed for CCND1 mRNA expression by Northern blot analysis. Results: In 13/57 (23%) cases, a 2-5 fold amplification of CCND1 was found. CCND1 mRNA expression was higher in amplified than in non-amplified tumors and supported an association between CCND1 amplification and increased expression of CCND1 mRNA. No correlation was found between CCND1 amplification or CCND1 expression and clinical or pathological parameters. However, analysis of risk factor exposure revealed that patients with greater tobacco exposure were more likely to have tumors with CCND1 amplification (p = .037). Also, tobacco exposure was correlated with CCND1 expression in tumors. Conclusion: Tobacco exposure is a well-known risk factor for HNSCC. CCND1 amplification and alterations in expression may be causally related to tobacco carcinogen exposure and lead to a loss of cell cycle regulation.

Original languageEnglish (US)
Pages (from-to)512-521
Number of pages10
JournalHead and Neck
Volume18
Issue number6
StatePublished - Nov 1 1996

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Cyclin D1
Tobacco
Neoplasms
Carcinoma, squamous cell of head and neck
Messenger RNA
bcl-1 Genes
Cell Cycle Proteins
Southern Blotting
Northern Blotting
Carcinogens
Cell Cycle

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Davidson, B. J., Lydiatt, W. M., Abate, M. P., Schantz, S. P., & Chaganti, R. S. K. (1996). Cyclin D1 abnormalities and tobacco exposure in head and neck squamous cell carcinoma. Head and Neck, 18(6), 512-521.

Cyclin D1 abnormalities and tobacco exposure in head and neck squamous cell carcinoma. / Davidson, Bruce J.; Lydiatt, William Michael; Abate, Megan P.; Schantz, Stimson P.; Chaganti, R. S.K.

In: Head and Neck, Vol. 18, No. 6, 01.11.1996, p. 512-521.

Research output: Contribution to journalArticle

Davidson, BJ, Lydiatt, WM, Abate, MP, Schantz, SP & Chaganti, RSK 1996, 'Cyclin D1 abnormalities and tobacco exposure in head and neck squamous cell carcinoma', Head and Neck, vol. 18, no. 6, pp. 512-521.
Davidson BJ, Lydiatt WM, Abate MP, Schantz SP, Chaganti RSK. Cyclin D1 abnormalities and tobacco exposure in head and neck squamous cell carcinoma. Head and Neck. 1996 Nov 1;18(6):512-521.
Davidson, Bruce J. ; Lydiatt, William Michael ; Abate, Megan P. ; Schantz, Stimson P. ; Chaganti, R. S.K. / Cyclin D1 abnormalities and tobacco exposure in head and neck squamous cell carcinoma. In: Head and Neck. 1996 ; Vol. 18, No. 6. pp. 512-521.
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