CXCL10 inhibits viral replication through recruitment of natural killer cells in coxsackievirus B3-Induced myocarditis

Ji Yuan, Zhen Liu, Travis Lim, Huifang Zhang, Jianqing He, Elizabeth Walker, Courtney Shier, Yinjing Wang, Yue Su, Alhousseynou Sall, Bruce Mcmanus, Decheng Yang

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Coxsackievirus (CV)B3 is the primary cause of viral myocarditis. We previously observed CXC chemokine ligand 10 (CXCL10) upregulation in the myocardium early in infection. However, the impact of CXCL10 in CVB3-induced myocarditis is unknown. Using isolated primary mouse cardiomyocytes we demonstrated for the first time that cardiomyocytes can express CXCL10 on interferon-γ stimulation. To explore the role of CXCL10 in CVB3-induced myocarditis, both CXCL10 transgenic and knockout mice were used. Following CVB3 challenges, the viral titer in the hearts inversely correlated with the levels of CXCL10 at early phase of infection before visible immune infiltration. Furthermore, as compared with the control mice, the decreased virus titers in the CXCL10 transgenic mouse hearts led to less cardiac damage and better cardiac function and vice verse in the knockout mice. This antiviral ability of CXCL10 might be through recruitment of natural killer (NK) cells to the heart and increased interferon-γ expression early in infection. At day 7 postinfection, with massive influx of mononuclear cells the expression of CXCL10 enhanced the infiltration of CXCR3 cells, CD4, and CD8 T cells, as well as the expression of associated inflammatory cytokines. However, the augmented accumulation of these immune cells and associated cytokines failed to alter the viral clearance and mice survival. These results suggest the protective role of CXCL10 during the early course of CVB3 infection, which is attributed to the recruitment of NK cells. Nonetheless, CXCL10-directed chemoattractant effect is not sufficient for host to clear the virus in the heart.

Original languageEnglish (US)
Pages (from-to)628-638
Number of pages11
JournalCirculation Research
Volume104
Issue number5
DOIs
StatePublished - Mar 13 2009

Fingerprint

CXC Chemokines
Enterovirus
Myocarditis
Natural Killer Cells
Ligands
Infection
Cardiac Myocytes
Knockout Mice
Interferons
Transgenic Mice
Cytokines
Chemotactic Factors
Viral Load
Antiviral Agents
Myocardium
Up-Regulation

Keywords

  • Cardiomyocytes
  • Chemokine CXCL 10
  • Coxsackievirus
  • Myocarditis
  • Natural killer cells

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

CXCL10 inhibits viral replication through recruitment of natural killer cells in coxsackievirus B3-Induced myocarditis. / Yuan, Ji; Liu, Zhen; Lim, Travis; Zhang, Huifang; He, Jianqing; Walker, Elizabeth; Shier, Courtney; Wang, Yinjing; Su, Yue; Sall, Alhousseynou; Mcmanus, Bruce; Yang, Decheng.

In: Circulation Research, Vol. 104, No. 5, 13.03.2009, p. 628-638.

Research output: Contribution to journalArticle

Yuan, J, Liu, Z, Lim, T, Zhang, H, He, J, Walker, E, Shier, C, Wang, Y, Su, Y, Sall, A, Mcmanus, B & Yang, D 2009, 'CXCL10 inhibits viral replication through recruitment of natural killer cells in coxsackievirus B3-Induced myocarditis', Circulation Research, vol. 104, no. 5, pp. 628-638. https://doi.org/10.1161/CIRCRESAHA.108.192179
Yuan, Ji ; Liu, Zhen ; Lim, Travis ; Zhang, Huifang ; He, Jianqing ; Walker, Elizabeth ; Shier, Courtney ; Wang, Yinjing ; Su, Yue ; Sall, Alhousseynou ; Mcmanus, Bruce ; Yang, Decheng. / CXCL10 inhibits viral replication through recruitment of natural killer cells in coxsackievirus B3-Induced myocarditis. In: Circulation Research. 2009 ; Vol. 104, No. 5. pp. 628-638.
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AU - Walker, Elizabeth

AU - Shier, Courtney

AU - Wang, Yinjing

AU - Su, Yue

AU - Sall, Alhousseynou

AU - Mcmanus, Bruce

AU - Yang, Decheng

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AB - Coxsackievirus (CV)B3 is the primary cause of viral myocarditis. We previously observed CXC chemokine ligand 10 (CXCL10) upregulation in the myocardium early in infection. However, the impact of CXCL10 in CVB3-induced myocarditis is unknown. Using isolated primary mouse cardiomyocytes we demonstrated for the first time that cardiomyocytes can express CXCL10 on interferon-γ stimulation. To explore the role of CXCL10 in CVB3-induced myocarditis, both CXCL10 transgenic and knockout mice were used. Following CVB3 challenges, the viral titer in the hearts inversely correlated with the levels of CXCL10 at early phase of infection before visible immune infiltration. Furthermore, as compared with the control mice, the decreased virus titers in the CXCL10 transgenic mouse hearts led to less cardiac damage and better cardiac function and vice verse in the knockout mice. This antiviral ability of CXCL10 might be through recruitment of natural killer (NK) cells to the heart and increased interferon-γ expression early in infection. At day 7 postinfection, with massive influx of mononuclear cells the expression of CXCL10 enhanced the infiltration of CXCR3 cells, CD4, and CD8 T cells, as well as the expression of associated inflammatory cytokines. However, the augmented accumulation of these immune cells and associated cytokines failed to alter the viral clearance and mice survival. These results suggest the protective role of CXCL10 during the early course of CVB3 infection, which is attributed to the recruitment of NK cells. Nonetheless, CXCL10-directed chemoattractant effect is not sufficient for host to clear the virus in the heart.

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