Current progress in the production of recombinant human fibrinogen in the milk of transgenic animals

Stephen P. Butler, Kevin Van Cott, Anuradha Subrumanian, Francis C. Gwazduaskas, William H. Velander

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The mammary gland of transgenic animals has several advantages for production of heterologous proteins including a high cell density that results in high concentrations of secreted protein. While the mammary gland appears to be able to secrete fully assembled recombinant human fibrinogen (rhfib) at 0.1 to 5 g/l levels, some unassembled rhfib chains are also secreted, Presently, the relationship between unassembled rhfib and the coordinated translation of each nascent rhfib polypeptide in the mammary epithelia is unknown. The secretion of fully and partially assembled rhfib is widely variable among mammalian cell lines and where previously no cell line has been shown to secrete beta chain alone. We have observed that mammary epithelia can secrete BP chain into milk as well as immature forms of other recombinant proteins, suggesting it likely uses a different secretory pathway than does the liver. This difference in secretory behavior is possibly due to the natural design of milk, where the precise regulation of post translational modifications and intracellular pools of nascent polypeptides needed to achieve fibrinogen assembly may be less important to fulfill the nutritional function of most milk proteins.

Original languageEnglish (US)
Pages (from-to)537-542
Number of pages6
JournalThrombosis and Haemostasis
Volume78
Issue number1
StatePublished - Jul 1 1997

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Genetically Modified Animals
Fibrinogen
Milk
Human Mammary Glands
Breast
Epithelium
Cell Line
Peptides
Milk Proteins
Secretory Pathway
Post Translational Protein Processing
Recombinant Proteins
Proteins
Cell Count
Liver

ASJC Scopus subject areas

  • Hematology

Cite this

Current progress in the production of recombinant human fibrinogen in the milk of transgenic animals. / Butler, Stephen P.; Van Cott, Kevin; Subrumanian, Anuradha; Gwazduaskas, Francis C.; Velander, William H.

In: Thrombosis and Haemostasis, Vol. 78, No. 1, 01.07.1997, p. 537-542.

Research output: Contribution to journalArticle

Butler, Stephen P. ; Van Cott, Kevin ; Subrumanian, Anuradha ; Gwazduaskas, Francis C. ; Velander, William H. / Current progress in the production of recombinant human fibrinogen in the milk of transgenic animals. In: Thrombosis and Haemostasis. 1997 ; Vol. 78, No. 1. pp. 537-542.
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