Curcumin-encapsulating nanogels as an effective anticancer formulation for intracellular uptake

Anna Reeves, Serguei V. Vinogradov, Phil Morrissey, Mitchell Chernin, Mansoor M. Ahmed

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Nanoscale drug delivery systems represent an attractive strategy to improve both the efficacy and safety of anticancer drugs. In this work, we describe nanoformulation of curcumin, a most potent natural anticancer compound capable of killing cancer cells while sparing the normal tissues. Since curcumin is a natural hydrophobic polyphenol, it has a low aqueous solubility and bioavailability, which are challenging to its therapeutic efficacy. We developed and evaluated a novel colloidal nanogel carrier for encapsulation of curcumin to increase its solubility and cytotoxicity. Amphiphilic Poloxamer-cationic network in the nanogel NG127 was designed to efficiently encapsulate curcumin. Homogenous drug complexes were obtained with 20-25% content of curcumin and the particle size of ca. 150 nm. Using ImageStream multispectral imaging flow cytometry, we demonstrated that the curcumin-nanogel formulation (C-NG) was readily internalized into MDA-231 breast cancer cells. A real-time cell growth electronic sensing assay was used to measure proliferation responses of various breast cancer cells to C-NG treatments. Our results indicated that the C-NG formulation was 70-85% more effective in inhibiting growth, at concentrations lower than IC50 of free curcumin. This was also confirmed morphologically by modified acridine orange/ethidium bromide staining and fluorescent microscopy. Importantly, nanocarrier NG127 alone displayed practically no cytotoxicity. We conclude that nanogel carriers offer an innovative way to encapsulate curcumin and to obtain more effective anticancer therapeutics than curcumin alone with a potential to specific tumor targeting, such as using antibodies against surface receptors specific to breast cancer cells.

Original languageEnglish (US)
Pages (from-to)25-40
Number of pages16
JournalMolecular and Cellular Pharmacology
Volume7
Issue number3
DOIs
StatePublished - 2015

Fingerprint

Curcumin
Breast Neoplasms
Solubility
NanoGel
Poloxamer
Acridine Orange
Ethidium
Polyphenols
Drug Delivery Systems
Growth
Particle Size
Pharmaceutical Preparations
Biological Availability
Inhibitory Concentration 50
Microscopy
Neoplasms
Flow Cytometry
Therapeutics
Staining and Labeling

Keywords

  • Anticancer drugs and breast cancer cells
  • Curcumin
  • Nanogels

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Molecular Biology

Cite this

Curcumin-encapsulating nanogels as an effective anticancer formulation for intracellular uptake. / Reeves, Anna; Vinogradov, Serguei V.; Morrissey, Phil; Chernin, Mitchell; Ahmed, Mansoor M.

In: Molecular and Cellular Pharmacology, Vol. 7, No. 3, 2015, p. 25-40.

Research output: Contribution to journalArticle

Reeves, Anna ; Vinogradov, Serguei V. ; Morrissey, Phil ; Chernin, Mitchell ; Ahmed, Mansoor M. / Curcumin-encapsulating nanogels as an effective anticancer formulation for intracellular uptake. In: Molecular and Cellular Pharmacology. 2015 ; Vol. 7, No. 3. pp. 25-40.
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