Cultured lung fibroblasts from ovalbumin-challenged "asthmatic" mice differ functionally from normal

Hisatoshi Sugiura, Xiang-de Liu, Fenghai Duan, Shin Kawasaki, Shinsaku Togo, Koichiro Kamio, Qi Wang Xing, Lijun Mao, Youngsoo Ahn, Ronald F. Ertl, Tom W. Bargar, Abdo Berro, Thomas B. Casale, Stephen I. Rennard

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Asthmatic airway remodeling is characterized by goblet cell hyperplasia, angiogenesis, smooth muscle hypertrophy, and subepithelial fibrosis. This study evaluated whether acquired changes in fibroblast phenotype could contribute to this remodeling. Airway and parenchymal fibroblasts from control or chronically ovalbumin (OVA)-sensitized and challenged "asthmatic" mice were assessed for several functions related to repair and remodeling ± exogenous transforming growth factor (TGF)-β. All OVA-challenged mouse fibroblasts demonstrated augmented gel contraction (P < 0.05) and Chemotaxis (P < 0.05); increased TGF-β 1 (P < 0.05), fibronectin (P < 0.05), and vascular endothelial growth factor (P < 0.05) release; and expressed more α-smooth muscle actin (P < 0.05). TGF- aβ 1 stimulated both control and asthmatic fibroblasts, which retained all differences from control fibroblasts for all features(P < 0.05, all comparisons). Parenchymal fibroblasts proliferated more rapidly (P < 0.05), while airway fibroblasts proliferated similarly compared with control fibroblasts (P = 0.25). Thus, in this animal model, OVA-challenged mouse fibroblasts acquire a distinct phenotype that differs from control fibroblasts. The augmented profibrotic activity and mediator release of asthmatic fibroblasts could contribute to airway remodeling in asthma.

Original languageEnglish (US)
Pages (from-to)424-430
Number of pages7
JournalAmerican journal of respiratory cell and molecular biology
Volume37
Issue number4
DOIs
StatePublished - Oct 1 2007

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Ovalbumin
Fibroblasts
Lung
Transforming Growth Factors
Airway Remodeling
Smooth Muscle
Muscle
Phenotype
Goblet Cells
Chemotaxis
Fibronectins
Hypertrophy
Vascular Endothelial Growth Factor A
Hyperplasia
Actins
Animals
Fibrosis
Repair
Asthma
Animal Models

Keywords

  • Fibroblast
  • Mouse model
  • Phenotype
  • Remodeling

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Cultured lung fibroblasts from ovalbumin-challenged "asthmatic" mice differ functionally from normal. / Sugiura, Hisatoshi; Liu, Xiang-de; Duan, Fenghai; Kawasaki, Shin; Togo, Shinsaku; Kamio, Koichiro; Xing, Qi Wang; Mao, Lijun; Ahn, Youngsoo; Ertl, Ronald F.; Bargar, Tom W.; Berro, Abdo; Casale, Thomas B.; Rennard, Stephen I.

In: American journal of respiratory cell and molecular biology, Vol. 37, No. 4, 01.10.2007, p. 424-430.

Research output: Contribution to journalArticle

Sugiura, H, Liu, X, Duan, F, Kawasaki, S, Togo, S, Kamio, K, Xing, QW, Mao, L, Ahn, Y, Ertl, RF, Bargar, TW, Berro, A, Casale, TB & Rennard, SI 2007, 'Cultured lung fibroblasts from ovalbumin-challenged "asthmatic" mice differ functionally from normal', American journal of respiratory cell and molecular biology, vol. 37, no. 4, pp. 424-430. https://doi.org/10.1165/rcmb.2007-0089OC
Sugiura, Hisatoshi ; Liu, Xiang-de ; Duan, Fenghai ; Kawasaki, Shin ; Togo, Shinsaku ; Kamio, Koichiro ; Xing, Qi Wang ; Mao, Lijun ; Ahn, Youngsoo ; Ertl, Ronald F. ; Bargar, Tom W. ; Berro, Abdo ; Casale, Thomas B. ; Rennard, Stephen I. / Cultured lung fibroblasts from ovalbumin-challenged "asthmatic" mice differ functionally from normal. In: American journal of respiratory cell and molecular biology. 2007 ; Vol. 37, No. 4. pp. 424-430.
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