Cultivar-dependent expression of a maize lipoxygenase responsive to seed infesting fungi

R. A. Wilson, H. W. Gardner, N. P. Keller

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Maize kernels are highly susceptible to Aspergillus spp. infection and aflatoxin (AF) contamination. Fatty acid signaling molecules appear to mediate the plant-fungal interaction by affecting the growth, development, and AF production of the fungus. In particular, fatty acid derivatives of the plant lipoxygenase (LOX) pathway are implicated in the Aspergillus spp.-seed interaction. The 9(S)-hydroperoxide derivative of linoleic acid promotes transcription of AF genes, whereas the 13(S)-hydroperoxide derivative decreases AF gene expression and production; both are sporulation factors. Our goal was to identify LOX genes responsive to Aspergillus spp. colonization and determine their specificities, 9(S)- or 13(S)-. Screening maize LOX expressed sequence tags (ESTs) identified one clone, cssap 92, which is highly expressed in Aspergillus spp.-infected seed susceptible to AF contamination and repressed in lines with resistance to AF contamination. The accumulation of cssap 92 transcript was similar during Fusarium spp. infection. The cDNA clone has 94% identity to the previously described L2 LOX gene from maize. Product-specificity analysis of the CSSAP 92 protein shows that it preferentially adds oxygen to carbon 9 of linoleic acid. Because 9(S)-hydroperoxy linoleic acid has been implicated as an aflatoxin-signaling molecule, it is possible that cssap 92 could be used as a biomarker that is indicative of AF resistance in maize lines.

Original languageEnglish (US)
Pages (from-to)980-987
Number of pages8
JournalMolecular Plant-Microbe Interactions
Volume14
Issue number8
DOIs
StatePublished - Jan 1 2001

Fingerprint

Aflatoxins
Lipoxygenase
lipoxygenase
Fungi
aflatoxins
Zea mays
Seed
Seeds
fungi
corn
Aspergillus
cultivars
seeds
Linoleic Acid
linoleic acid
Contamination
Genes
hydroperoxides
Derivatives
Hydrogen Peroxide

Keywords

  • Aspergillus flavus
  • Fusarium verticillioides
  • Plant defense gene
  • Zea mays

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Cultivar-dependent expression of a maize lipoxygenase responsive to seed infesting fungi. / Wilson, R. A.; Gardner, H. W.; Keller, N. P.

In: Molecular Plant-Microbe Interactions, Vol. 14, No. 8, 01.01.2001, p. 980-987.

Research output: Contribution to journalArticle

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