Crystals of ternary protein-DNA complexes composed of DNA-binding domains of c-Myb or v-Myb, C/EBPα or C/EBPβ and tom-1A promoter fragment

Tahir H Tahirov, M. Sasaki, T. Inoue-Bungo, A. Fujikawa, K. Sato, T. Kumasaka, M. Yamamoto, K. Ogata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

c-Myb and the C/EBP family are transcriptional regulatory factors that act in concert to regulate the expression of myeloid-specific genes, v-Myb encoded by avian myeloblastosis virus (AMV) is a mutated form of c-Myb that contains point mutations which disrupt the cooperation with C/EBPs. To understand the mechanism of the transcriptional synergy between c-Myb and C/EBPs and the effect of the v-Myb mutations on that synergy, knowledge based on their three-dimensional structures is essential. Crystals of ternary complexes, in which various combinations of the DNA-binding domains of c-Myb or v-Myb and C/EBPα or C/EBPβ are bound to a DNA fragment from tom-1A promoter, were obtained by the vapour-diffusion method. Complete diffraction data sets were obtained from each native crystal and two types of iodine-derivative crystals. A three-wavelength MAD data set was also obtained from a bromine-derivative crystal.

Original languageEnglish (US)
Pages (from-to)1655-1658
Number of pages4
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number11
DOIs
StatePublished - Nov 20 2001

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Ternary Complex Factors
deoxyribonucleic acid
myb Genes
Avian Myeloblastosis Virus
fragments
proteins
Bromine
Crystals
DNA
mutations
Point Mutation
Iodine
crystals
Proteins
Derivatives
Mutation
viruses
bromine
Viruses
genes

ASJC Scopus subject areas

  • Structural Biology

Cite this

Crystals of ternary protein-DNA complexes composed of DNA-binding domains of c-Myb or v-Myb, C/EBPα or C/EBPβ and tom-1A promoter fragment. / Tahirov, Tahir H; Sasaki, M.; Inoue-Bungo, T.; Fujikawa, A.; Sato, K.; Kumasaka, T.; Yamamoto, M.; Ogata, K.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 57, No. 11, 20.11.2001, p. 1655-1658.

Research output: Contribution to journalArticle

Tahirov, Tahir H ; Sasaki, M. ; Inoue-Bungo, T. ; Fujikawa, A. ; Sato, K. ; Kumasaka, T. ; Yamamoto, M. ; Ogata, K. / Crystals of ternary protein-DNA complexes composed of DNA-binding domains of c-Myb or v-Myb, C/EBPα or C/EBPβ and tom-1A promoter fragment. In: Acta Crystallographica Section D: Biological Crystallography. 2001 ; Vol. 57, No. 11. pp. 1655-1658.
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abstract = "c-Myb and the C/EBP family are transcriptional regulatory factors that act in concert to regulate the expression of myeloid-specific genes, v-Myb encoded by avian myeloblastosis virus (AMV) is a mutated form of c-Myb that contains point mutations which disrupt the cooperation with C/EBPs. To understand the mechanism of the transcriptional synergy between c-Myb and C/EBPs and the effect of the v-Myb mutations on that synergy, knowledge based on their three-dimensional structures is essential. Crystals of ternary complexes, in which various combinations of the DNA-binding domains of c-Myb or v-Myb and C/EBPα or C/EBPβ are bound to a DNA fragment from tom-1A promoter, were obtained by the vapour-diffusion method. Complete diffraction data sets were obtained from each native crystal and two types of iodine-derivative crystals. A three-wavelength MAD data set was also obtained from a bromine-derivative crystal.",
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AU - Sasaki, M.

AU - Inoue-Bungo, T.

AU - Fujikawa, A.

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AU - Kumasaka, T.

AU - Yamamoto, M.

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