Crystallization and preliminary X-ray study of H2-proteinase from the venom of Trimeresurus flavoviridis

Takashi Kumasaka, Hiroyuki Takeya, Masaki Yamamoto, Yoshio Yamakawa, Tamotsu Omori-satoh, Hideaki Moriyama, Nobuo Tanaka, Mamoru Sato, Yukiteru Katsube, Sadaaki Iwanaga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

H2-proteinase, a non-hemorrhagic metalloproteinase from the venom of Trimeresurus flavoviridis, has been crystallized by vapor diffusion from solutions containing ammonium sulfate. The crystals belong to the tetragonal space group, P41212 or P43212, with unit cell dimensions of a = b = 77.8 å and c = 82.3 å. The asymmetric unit contains one protein molecule. Diffraction data for a native crystal were collected up to 2.0 å resolution

Original languageEnglish (US)
Pages (from-to)929-930
Number of pages2
JournalJournal of Biochemistry
Volume117
Issue number5
DOIs
StatePublished - May 1995

Fingerprint

Venoms
Ammonium Sulfate
Metalloproteases
Crystallization
Crystal
X-Rays
X rays
Crystals
Unit
Ammonium
Diffraction
Proteins
Vapors
Molecules
Protein
Cell
H(2)-proteinase
aspergillopepsin II
Trimeresurus venoms
Peptide Hydrolases

Keywords

  • Crystallization
  • Metalloproteinase
  • Snake venom
  • X-ray diffraction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Crystallization and preliminary X-ray study of H2-proteinase from the venom of Trimeresurus flavoviridis. / Kumasaka, Takashi; Takeya, Hiroyuki; Yamamoto, Masaki; Yamakawa, Yoshio; Omori-satoh, Tamotsu; Moriyama, Hideaki; Tanaka, Nobuo; Sato, Mamoru; Katsube, Yukiteru; Iwanaga, Sadaaki.

In: Journal of Biochemistry, Vol. 117, No. 5, 05.1995, p. 929-930.

Research output: Contribution to journalArticle

Kumasaka, T, Takeya, H, Yamamoto, M, Yamakawa, Y, Omori-satoh, T, Moriyama, H, Tanaka, N, Sato, M, Katsube, Y & Iwanaga, S 1995, 'Crystallization and preliminary X-ray study of H2-proteinase from the venom of Trimeresurus flavoviridis', Journal of Biochemistry, vol. 117, no. 5, pp. 929-930. https://doi.org/10.1093/oxfordjournals.jbchem.a124820
Kumasaka, Takashi ; Takeya, Hiroyuki ; Yamamoto, Masaki ; Yamakawa, Yoshio ; Omori-satoh, Tamotsu ; Moriyama, Hideaki ; Tanaka, Nobuo ; Sato, Mamoru ; Katsube, Yukiteru ; Iwanaga, Sadaaki. / Crystallization and preliminary X-ray study of H2-proteinase from the venom of Trimeresurus flavoviridis. In: Journal of Biochemistry. 1995 ; Vol. 117, No. 5. pp. 929-930.
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