Crystallization and preliminary X-ray studies of isocitrate dehydrogenase from thermus thermophilus hb8

Masato Ohzeki, Takuro Yaoi, Hideaki Moriyama, Tairo Oshima, Nobuo Tanaka

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4 Scopus citations

Abstract

Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant. Pillar-like crystals of about 0.6×0.6×0.3 mm3 were obtained. Analysis of a series of oscillation photographs indicated that the orthorhombic crystals belonged to the I222 or I212121 space group with unit cell dimensions of a =100.1 Å, b=150.4 A, and c=87.4 Å. Intensity data were collected up to 2.5 Å resolution.

Original languageEnglish (US)
Pages (from-to)679-680
Number of pages2
JournalJournal of Biochemistry
Volume118
Issue number4
DOIs
Publication statusPublished - Oct 1995

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Keywords

  • Crystallization
  • Isocitrate dehydrogenase
  • Synchrotron
  • Thermus thermophilus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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