Crystallization and preliminary X-ray studies of isocitrate dehydrogenase from thermus thermophilus hb8

Masato Ohzeki, Takuro Yaoi, Hideaki Moriyama, Tairo Oshima, Nobuo Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant. Pillar-like crystals of about 0.6×0.6×0.3 mm3 were obtained. Analysis of a series of oscillation photographs indicated that the orthorhombic crystals belonged to the I222 or I212121 space group with unit cell dimensions of a =100.1 Å, b=150.4 A, and c=87.4 Å. Intensity data were collected up to 2.5 Å resolution.

Original languageEnglish (US)
Pages (from-to)679-680
Number of pages2
JournalJournal of Biochemistry
Volume118
Issue number4
DOIs
StatePublished - Jan 1 1995

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Thermus thermophilus
Isocitrate Dehydrogenase
Crystallization
X-Rays
Bacteria
X rays
Crystals
Vapors
Polyethylene Glycol 6000

Keywords

  • Crystallization
  • Isocitrate dehydrogenase
  • Synchrotron
  • Thermus thermophilus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Crystallization and preliminary X-ray studies of isocitrate dehydrogenase from thermus thermophilus hb8. / Ohzeki, Masato; Yaoi, Takuro; Moriyama, Hideaki; Oshima, Tairo; Tanaka, Nobuo.

In: Journal of Biochemistry, Vol. 118, No. 4, 01.01.1995, p. 679-680.

Research output: Contribution to journalArticle

Ohzeki, Masato ; Yaoi, Takuro ; Moriyama, Hideaki ; Oshima, Tairo ; Tanaka, Nobuo. / Crystallization and preliminary X-ray studies of isocitrate dehydrogenase from thermus thermophilus hb8. In: Journal of Biochemistry. 1995 ; Vol. 118, No. 4. pp. 679-680.
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