A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.
|Original language||English (US)|
|Number of pages||2|
|Journal||Journal of Biochemistry|
|Publication status||Published - Jan 1991|
ASJC Scopus subject areas
- Molecular Biology