Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

Ko Onodera, Hideaki Moriyama, Akio Takenaka, Nobuo Tanaka, Nobuko Akutsu, Masahito Muro, Tairo Oshima, Katsumi Imada, Mamoru Sato, Yukiteru Katsube

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Abstract

A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.

Original languageEnglish (US)
Pages (from-to)1-2
Number of pages2
JournalJournal of Biochemistry
Volume109
Issue number1
DOIs
Publication statusPublished - Jan 1991

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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