Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

Ko Onodera, Hideaki Moriyama, Akio Takenaka, Nobuo Tanaka, Nobuko Akutsu, Masahito Muro, Tairo Oshima, Katsumi Imada, Mamoru Sato, Yukiteru Katsube

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.

Original languageEnglish (US)
Pages (from-to)1-2
Number of pages2
JournalJournal of Biochemistry
Volume109
Issue number1
DOIs
StatePublished - Jan 1 1991

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Thermus thermophilus
Bacilli
Crystallization
Bacillus subtilis
3-Isopropylmalate Dehydrogenase
Enzymes
Genes
X-Rays
Gene
X rays
Escherichia coli
Escherichia Coli
Crystal
Coding
Crystals
beta-isopropylmalate
Oxidoreductases

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase. / Onodera, Ko; Moriyama, Hideaki; Takenaka, Akio; Tanaka, Nobuo; Akutsu, Nobuko; Muro, Masahito; Oshima, Tairo; Imada, Katsumi; Sato, Mamoru; Katsube, Yukiteru.

In: Journal of Biochemistry, Vol. 109, No. 1, 01.01.1991, p. 1-2.

Research output: Contribution to journalArticle

Onodera, Ko ; Moriyama, Hideaki ; Takenaka, Akio ; Tanaka, Nobuo ; Akutsu, Nobuko ; Muro, Masahito ; Oshima, Tairo ; Imada, Katsumi ; Sato, Mamoru ; Katsube, Yukiteru. / Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase. In: Journal of Biochemistry. 1991 ; Vol. 109, No. 1. pp. 1-2.
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AU - Takenaka, Akio

AU - Tanaka, Nobuo

AU - Akutsu, Nobuko

AU - Muro, Masahito

AU - Oshima, Tairo

AU - Imada, Katsumi

AU - Sato, Mamoru

AU - Katsube, Yukiteru

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